Using Convolutional Neural Network as a Statistical Algorithm to Explore the Therapeutic Effect of Insulin Liposomes on Corneal Inflammation

作者全名："Liao, Yukun; Jiang, Huiting; Du, Yangrui; Xiong, Xiaojing; Zhang, Yu; Du, Zhiyu"

作者地址："[Liao, Yukun; Jiang, Huiting; Du, Yangrui; Xiong, Xiaojing; Zhang, Yu; Du, Zhiyu] Chongqing Med Univ, Affiliated Hosp 2, Dept Ophthalmol, Chongqing 400010, Peoples R China; [Liao, Yukun; Jiang, Huiting; Xiong, Xiaojing] Chongqing Med Univ, Affiliated Hosp 2, Chongqing Key Lab Ultrasound Mol Imaging, Chongqing 400010, Peoples R China"

通信作者："Du, ZY (通讯作者)，Chongqing Med Univ, Affiliated Hosp 2, Dept Ophthalmol, Chongqing 400010, Peoples R China."

来源：COMPUTATIONAL INTELLIGENCE AND NEUROSCIENCE

ESI学科分类：NEUROSCIENCE & BEHAVIOR

WOS号：WOS:000855557500002

JCR分区：Q3

影响因子：3.12

年份：2022

卷号：2022

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：　

摘要："Aiming at the disadvantages of easy recurrence of keratitis, difficult eradication by surgery, and easy bacterial resistance, insulin-loaded liposomes were prepared, and convolutional neural network was used as a statistical algorithm to build SD rat corneal inflammation model and study insulin-loaded liposomes, alleviating effect on corneal inflammatory structure in SD rats. The INS/PFOB@LIP was developed by means of thin-film dispersive phacoemulsification, its structure was monitored using a transmission electron microscope, particle size and appearance potential were monitored using a Malvern particle sizer, and ultraviolet consumption spectrum was monitored using a UV spectrophotometer. The encapsulation rate, drug loading, and distribution of insulin liposomes in rat corneal inflammatory model were measured and calculated. The cytotoxicity of liposome materials was evaluated by CCK-8 assay, and the toxic effects of insulin and insulin liposomes on cells were detected. The cornea of SD rats was burned with NaOH solution (1 mol/L), and the SD rat corneal inflammation model was created. The insulin liposome was applied to the corneal inflammation model, and the therapeutic effect of insulin liposome on corneal inflammation was evaluated by slit lamp, corneal immunohistochemistry, corneal HE staining, and corneal Sirius red staining. Insulin-loaded liposomes were successfully constructed with an average particle size of (130.69 +/- 3.87) nm and a surface potential of (-38.24 +/- 2.57) mV. The encapsulation rate of insulin liposomes was (48.89 +/- 1.24)%, and the drug loading rate was (24.45 +/- 1.24)%. The SD rat corneal inflammation model was successfully established. After insulin liposome treatment, the staining area of corneal fluorescein sodium was significantly reduced, the corneal epithelium was significantly thickened, the content of corneal collagen was increased, the expression of inflammatory factors was significantly reduced, and new blood vessels (corneal neovascularization, CNV) growth was inhibited."

基金机构：Department of Ultrasound at the Second Affiliated Hospital of Chongqing Medical University; Natural Science Foundation of Chongqing City [CQYC20200303112]

基金资助正文：The authors thank the Department of Ultrasound at the Second Affiliated Hospital of Chongqing Medical University and Chongqing Mingda Ophthalmology for the support of experiments. This work was supported by the Natural Science Foundation of Chongqing City (CQYC20200303112).