An ultra-sensitive dual-signal ratiometric electrochemical aptasensor based on functionalized MOFs for detection of HER2

作者全名："Xu, Ying; Zhang, Ya; Li, Ning; Yang, Mei; Xiang, Tingxiu; Huo, Danqun; Qiu, Zhu; Yang, Li; Hou, Changjun"

作者地址："[Xu, Ying; Zhang, Ya; Li, Ning; Yang, Mei; Huo, Danqun; Yang, Li; Hou, Changjun] Chongqing Univ, Key Lab Biorheol Sci & Technol, Minist Educ, Bioengn Coll, Chongqing 400044, Peoples R China; [Qiu, Zhu] Chongqing Med Univ, Chongqing Key Lab Mol Oncol & Epigenet, Affiliated Hosp 1, Chongqing 400016, Peoples R China; [Xiang, Tingxiu] Chongqing Univ, Chongqing Key Lab Translat Res Canc Metastasis & I, Canc Hosp, Chongqing 400030, Peoples R China; [Hou, Changjun] Chongqing Univ, Sch Microelect & Commun Engn, Chongqing Key Lab Biopercept & Intelligent Informa, Chongqing 400044, Peoples R China"

通信作者："Yang, L; Hou, CJ (通讯作者)，Chongqing Univ, Key Lab Biorheol Sci & Technol, Minist Educ, Bioengn Coll, Chongqing 400044, Peoples R China.; Qiu, Z (通讯作者)，Chongqing Med Univ, Chongqing Key Lab Mol Oncol & Epigenet, Affiliated Hosp 1, Chongqing 400016, Peoples R China.; Hou, CJ (通讯作者)，Chongqing Univ, Sch Microelect & Commun Engn, Chongqing Key Lab Biopercept & Intelligent Informa, Chongqing 400044, Peoples R China."

来源：BIOELECTROCHEMISTRY

ESI学科分类：BIOLOGY & BIOCHEMISTRY

WOS号：WOS:000876731200004

JCR分区：Q1

影响因子：5

年份：2022

卷号：148

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：HER2; ZIF-67@Fc; AMNFs; ZIF-90@MB; Ratiometric electrochemical aptasensor

摘要："The dual-signal radiometric sensor can effectively reduce the difference between repeated detection and achieve higher accuracy, sensitivity and repeatability detection. In this work, we constructed a simple ratiometric electrochemical aptasensor based on functionalized ZIF-67 and ZIF-90 for sensitive detection of human epidermal growth factor receptor-2 (HER2). ZIF-67@Ferrocene (Fc)/antimonate nano flakes (AMNFs) as the capture probe has a large specific surface area and good conductivity, and have a strong adsorption capacity for aptamer single-stranded deoxyribonucleic acid (ssDNA). When the biomarker -HER2 interacts with aptamer ssDNA, it is easily desorbed from its surface. At the same time, ZIF-90@ methylene blue (MB) as the signal probe realizes one-step encapsulation of MB signal, which can avoid interference from external environment. When the target-HER2 exists, it is recognized by the capture probe, which leads to the decrease of the conductivity of the electrode. Under the action of the signal probe, the conductivity of the signal is recovered and the detection signal is amplified significantly. The designed ratiometric electrochemical aptasensor showed a wide linear range (0.5-1000 pg mL-1) and a low detection limit (155 fg mL-1) for HER2. Subsequently, it was applied to actual serum samples and showed acceptable applicability. It shows great potential for clinical screening and immediate detection of cancer biomarkers."

基金机构："Chongqing Science and Technology Commission, China [cstc2021jcyj-msxmX0608]; Sichuan Science and Technology Program [2022YFSY0013]; Graduate Scientific Research and Innovation Foundation of Chongqing, China [CYB22072]; National Facility for Translational Medicine (Shanghai) Open Research Found [TMSK-2021-113]; Chongqing Graduate Tutor Team Construction Project; Chongqing University's Large Equipment, China; Analytical and Testing Center of Chongqing University"

基金资助正文："This work was supported by the Chongqing Science and Technology Commission, China (cstc2021jcyj-msxmX0608), Sichuan Science and Technology Program (2022YFSY0013), Graduate Scientific Research and Innovation Foundation of Chongqing, China (Grant CYB22072), National Facility for Translational Medicine (Shanghai) Open Research Found (TMSK-2021-113), Chongqing Graduate Tutor Team Construction Project and Analytical and Testing Center of Chongqing University for SEM/TEM/the characterization of SEM/XRD/XPS/TEM and the sharing fund of Chongqing University's Large Equipment, China."