Predictive value of tumor-infiltrating lymphocytes detected by flow cytometry in colorectal cancer
作者全名："Hu, Yanping; Zhao, Jiuzhou; Shen, Yihang; Zhang, Chengjuan; Xia, Qingxin; Zhang, Guoqiang; Wang, Bo; Wei, Bing; Yu, Rentao; Ma, Jie; Guo, Yongjun"
作者地址："[Hu, Yanping; Zhao, Jiuzhou; Wang, Bo; Wei, Bing; Ma, Jie; Guo, Yongjun] Zhengzhou Univ, Affiliated Canc Hosp, Henan Canc Hosp, Dept Mol Pathol, Zhengzhou, Peoples R China; [Hu, Yanping; Zhao, Jiuzhou; Wang, Bo; Ma, Jie; Guo, Yongjun] Henan Key Lab Mol Pathol, Zhengzhou, Peoples R China; [Zhao, Jiuzhou] Zhengzhou Univ, Coll Basic Med Sci, Dept Microbiol & Immunol, Zhengzhou, Peoples R China; [Shen, Yihang] Suzhou Ninth Peoples Hosp, Cent Lab, Suzhou, Peoples R China; [Yu, Rentao] Chongqing Med Univ, Affiliated Hosp 1, Dept Dermatol, Chongqing, Peoples R China; [Zhang, Chengjuan; Ma, Jie] Zhengzhou Univ, Affiliated Canc Hosp, Ctr Repository, Zhengzhou, Peoples R China; [Xia, Qingxin] Zhengzhou Univ, Affiliated Canc Hosp, Henan Canc Hosp, Dept Pathol, Zhengzhou, Peoples R China; [Zhang, Guoqiang] Zhengzhou Univ, Affiliated Canc Hosp, Henan Canc Hosp, Dept Gen Surg, Zhengzhou, Peoples R China; [Yu, Rentao; Ma, Jie; Guo, Yongjun] Henan Canc Hosp, 126 Dongming Rd, Zhengzhou 450000, Peoples R China"
通信作者："Yu, RT; Ma, J; Guo, YJ (通讯作者)，Henan Canc Hosp, 126 Dongming Rd, Zhengzhou 450000, Peoples R China."
来源：INTERNATIONAL IMMUNOPHARMACOLOGY
ESI学科分类：PHARMACOLOGY & TOXICOLOGY
WOS号：WOS:000877709800003
JCR分区：Q1
影响因子：5.6
年份：2022
卷号：113
期号：
开始页：
结束页：
文献类型：Article
关键词：Colorectal cancer; Tumor-infiltrating lymphocytes; Flow cytometry; Disease-free survival
摘要："The high heterogeneity of tumor cells and the surrounding immune microenvironment affects the response to treatment in colorectal cancer (CRC) patients. Therefore, there is a need to identify new immune biomarkers to predict the treatment efficacy of CRC. This study aimed to explore the predictive value of tumor-infiltrating lymphocytes (TIL) for survival in CRC patients. Flow cytometry and gated analysis were performed to mea-sure the TILs in tissue samples obtained from 536 CRC patients. The COX regression analysis showed that the CD8 + CD279+ cells had the highest impact of all evaluated TILs on postoperative disease-free survival (DFS) (P < 0.05). The optimal CD8 + CD279+ cutoff point for the prediction of survival was 12.2%. The Kaplan-Meier analysis showed significantly higher DFS in the high CD8 + CD279+ group compared with the low CD8 + CD279+ group (P < 0.05). CD8 + CD279+ cells were associated with DFS in CRC patients with the KARS mutation, MSI/MMR, perineural invasion, and those treated with neoadjuvant chemotherapy and other chemotherapeutic treatments (P < 0.05). After the multivariate adjustment, the expression of CD8 + CD279+ remained an independent risk factor for DFS. Overall, the CD8 + CD279+ cells were identified as an independent prognostic factor in CRC patients and could be used as a potential marker for postoperative DFS."
基金机构："Joint Co-construction Project of Henan Medical Science and Technology Research Plan [LHGJ20210199]; Key R & D and promotion projects in Henan Province of 2020 (scientific and technical program) [222102310266, 212102310657]; Major public welfare projects in Henan Province [201300310400]"
基金资助正文："The work was funded by Joint Co-construction Project of Henan Medical Science and Technology Research Plan (NO. LHGJ20210199) , Key R & D and promotion projects in Henan Province of 2020 (scientific and technical program) (NO. 222102310266, 212102310657) , Major public welfare projects in Henan Province (201300310400) - Research and development of new technologies for tumor liquid biopsy and immunotherapy."
