An ultrasensitive electrochemical immunosensor for carcinoembryonic antigen detection based on two-dimensional PtPd/Cu-TCPP(Fe) nanocomposites
作者全名："Liu, Jie; Zhang, Zixuan; Li, Ying; Dong, Jie; Li, Chaorui"
作者地址："[Liu, Jie; Zhang, Zixuan; Li, Ying; Dong, Jie; Li, Chaorui] Chongqing Med Univ, Sch Publ Hlth, Chongqing 400016, Peoples R China"
通信作者："Li, CR (通讯作者)，Chongqing Med Univ, Sch Publ Hlth, Chongqing 400016, Peoples R China."
来源：ANALYTICAL AND BIOANALYTICAL CHEMISTRY
ESI学科分类：CHEMISTRY
WOS号：WOS:000882122600001
JCR分区：Q1
影响因子：4.3
年份：2022
卷号：
期号：
开始页：
结束页：
文献类型：Article; Early Access
关键词：PtPd/Cu-TCPP(Fe) nanocomposites; Silicon dioxide tag antibody; Sandwich-type electrochemical immunosensor; Carcinoembryonic antigen
摘要："Establishing an effective signal amplification strategy is the key to achieving sensitive detection of analytes by electrochemical immunoassay. In this work, a novel sandwich-type electrochemical immunosensor with dual-signal amplification was successfully constructed using PtPd/Cu-TCPP(Fe) as the sensing platform and mesoporous silicon dioxide as the signal amplifier. Firstly, two-dimensional wrinkled Cu-TCPP(Fe) nanomaterials loaded with PtPd nanoparticles have strong affinity for the immobilization of capture antibodies and can generate excellent electrochemical signals. Meanwhile, the mesoporous silicon dioxide with large steric hindrance was used as signal label to further improve the sensitivity of the immunosensor by increasing the difference of the current response signal. Under optimal experimental conditions, the electrochemical immunosensor exhibited a wide linear detection range from 0.1 pg/mL to 1.0 mu g/mL, with a detection limit as low as 0.166 fg/mL. The experimental results showed that the constructed immunosensor has a great application prospect in clinical biomarker detection."
基金机构："Natural Science Foundation of Chongqing, China [cstc2019jcyj-msxmX0121]"
基金资助正文："This work was supported by the Natural Science Foundation of Chongqing, China (No. cstc2019jcyj-msxmX0121)."
