Biocompatible engineered erythrocytes as plasmonic sensor initiators for high-sensitive screening of non-small cell lung cancer-derived exosomal miRNA in an integrated system

作者全名："Fan, Yunpeng; Wu, Wenwen; Xie, Ning; Huang, Yi; Wu, Haiping; Zhang, Juan; Guo, Xiaolan; Ding, Shijia; Guo, Bin"

作者地址："[Xie, Ning; Huang, Yi; Guo, Xiaolan; Guo, Bin] North Sichuan Med Coll, Dept Lab Med, Affiliated Hosp, Nanchong 637000, Sichuan, Peoples R China; [Fan, Yunpeng; Wu, Wenwen; Xie, Ning; Wu, Haiping; Guo, Xiaolan; Ding, Shijia; Guo, Bin] Chongqing Med Univ, Coll Lab Med, Key Lab Clin Lab Diagnost, Minist Educ, Chongqing 400016, Peoples R China; [Fan, Yunpeng; Zhang, Juan] Chongqing Hosp Tradit Chinese Med, Chongqing Key Lab Sichuan Chongqing Coconstruct Di, Chongqing 400021, Peoples R China; [Xie, Ning; Huang, Yi; Guo, Xiaolan; Guo, Bin] North Sichuan Med Coll, Res Ctr Translat Med, Dept Med Lab Sci, Nanchong 637000, Sichuan, Peoples R China"

通信作者："Guo, B (通讯作者)，North Sichuan Med Coll, Dept Lab Med, Affiliated Hosp, Nanchong 637000, Sichuan, Peoples R China.; Ding, SJ; Guo, B (通讯作者)，Chongqing Med Univ, Coll Lab Med, Key Lab Clin Lab Diagnost, Minist Educ, Chongqing 400016, Peoples R China."

来源：BIOSENSORS & BIOELECTRONICS

ESI学科分类：CHEMISTRY

WOS号：WOS:000888085200002

JCR分区：Q1

影响因子：10.7

年份：2023

卷号：219

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Exosomes; Engineered erythrocytes; Metal-organic frameworks; Quadruplet supramolecular dendrimer; Non-small cell lung cancer

摘要："The development of a holistic solution is crucial for exosome-based liquid biopsy, which is a significant advancement from basic research to clinical application for the early and non-invasive diagnosis of disease. However, the challenge of current technology is how to facilitate the separation and quickly connect with the detection. Herein, employing miRNA-155 as a target, a novel integrated concentration and determination system of exosomes (ICDSE) was fabricated for the targeted enrichment of exosomes from the plasma of patients with non-small cell lung cancer and instant downstream analysis of exosomal miRNA. This ICDSE consists of aptamerengineered erythrocytes with a supramolecular plasmonic biosensor. The streamlined analytical procedure benefited from engineered erythrocytes as an interlinkage, needlessly removing them before extracting total RNA due to the lack of nuclei. With the assistance of a specific aptamer and simple centrifugation, engineered erythrocytes achieved exceptional enrichment of exosomes within 30 min. The LOD of the biosensor for miR-155 approached 2.03 fM due to the substantially high refractive index of the quadruplet supramolecular dendrimer and zirconium metal-organic framework. Impressively, the developed ICDSE successfully isolated and evaluated exosomes from clinical specimens of patients with NSCLC, which can also be promising for other diseases with identifiable exosome signatures. Thus, our ICDSE is expected to have widespread biomedical applications, as it is economical and well-accepted."

基金机构："Science and Technology Project of Sichuan [2019YJ0392]; Science and Technology Project of Nanchong [18SXHZ0512]; Doctor foundation of North Sichuan Medical College [CBY19-QD01]; National Natural Science Foundation of China [82272436, 81873980, 82272432]; National Science and Technology Major Project of the Ministry of Science and Technology of China [2018ZX10732202]; Chongqing Natural Science Foundation [CSTB2022NSCQ-MSX0377]"

基金资助正文："This work was supported by Science and Technology Project of Sichuan (2019YJ0392) , Science and Technology Project of Nanchong (18SXHZ0512) , Doctor foundation of North Sichuan Medical College (CBY19-QD01) , National Natural Science Foundation of China (82272436, 81873980, 82272432) , National Science and Technology Major Project of the Ministry of Science and Technology of China (2018ZX10732202) , Chongqing Natural Science Foundation (CSTB2022NSCQ-MSX0377) ."