Decreased APOC1 expression inhibited cancer progression and was associated with better prognosis and immune microenvironment in esophageal cancer

作者全名："Guo, Qiang; Liu, Xiao-Li; Jiang, Ni; Zhang, Wen-Jun; Guo, Shao-Wen; Yang, Heng; Ji, Yan-Mei; Zhou, Jun; Guo, Jia-Long; Zhang, Jun; Liu, Hua-Song"

作者地址："[Guo, Qiang; Yang, Heng; Zhou, Jun; Guo, Jia-Long; Zhang, Jun; Liu, Hua-Song] Hubei Univ Med, Taihe Hosp, Dept Cardiothorac Surg, Shiyan, Hubei, Peoples R China; [Zhang, Wen-Jun; Guo, Shao-Wen] Hubei Univ Med, Taihe Hosp, Dept Med Ultrasound, Shiyan, Hubei, Peoples R China; [Ji, Yan-Mei] Hubei Univ Med, Taihe Hosp, Dept Crit Care Med, Shiyan, Hubei, Peoples R China; [Liu, Xiao-Li] Peoples Hosp Jianyang City, Dept Ultrasound, Jianyang, Sichuan, Peoples R China; [Jiang, Ni] Chongqing Med Univ, Dept Obstet & Gynecol, Women & Childrens Hosp, Chongqing, Peoples R China"

通信作者："Guo, JL; Zhang, J; Liu, HS (通讯作者)，Hubei Univ Med, Taihe Hosp, Dept Cardiothorac Surg, Shiyan, Hubei, Peoples R China."

来源：AMERICAN JOURNAL OF CANCER RESEARCH

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:000892363200003

JCR分区：Q2

影响因子：5.3

年份：2022

卷号：12

期号：11

开始页：4904

结束页：+

文献类型：Article

关键词：APOC1; ESCA; immune microenvironment; prognosis; biomarker

摘要："Several studies have demonstrated the involvement of apolipoprotein C1 (APOC1) in multiple cancers. However, the role of APOC1 in esophageal cancer (ESCA) has not been elucidated. Hence, we examined the expression of APOC1 in ESCA tissues acquired from The Cancer Genome Atlas (TCGA) database and clinical samples from our hospital. An investigation of the association of APOC1 with the clinicopathological characteristics, prognosis, and diagnosis of ESCA was carried out on the basis of survival, receiver operating characteristics, and correlation analyses. Gene ontology, KEGG analysis, and protein-protein interaction network showed that co-expressed APOC1 genes were involved in the functions, mechanisms, and action network. The effects of APOC1 expression on ESCA cells were explored using CCK-8, migration and invasion assays. The relationship between APOC1 expression and ESCA immune-infiltrating cells and cell markers were examined using correlation analysis. We found that APOC1 was overexpressed in TCGA ESCA tissues and the same was validated in clinical ESCA tissues, with the area under the curve for APOC1 being 0.887. Overexpression of APOC1 was associated with short overall survival, disease-specific of APOC1 expression significantly reduced the proliferation, migration, and invasion of ESCA cells. Furthermore, APOC1 expression positively correlated with the ESTIMATE, immune, and stromal scores in ESCA. Overexpression of APOC1 correlated with the tumor purity, B cells, T helper cells, natural killer cells, cytotoxic cells, and other immune cells. Moreover, APOC1 was involved in ESCA progression via T cell receptor, B cell receptor, and other immune signaling pathways. Thus, APOC1 overexpression is expected to be a biomarker for dismal prognosis and diagnosis of ESCA. Inhibition of APOC1 expression significantly reduced the proliferation, migration, and invasion of ESCA cells. Overexpression of APOC1 was associated with the immune microenvironment in ESCA. Thus, APOC1 may be an efficient biomarker for proper prognosis and diagnosis of ESCA."

基金机构：Health Commission of Hubei Province scientific research project; Hubei Chen Xiaoping science and Technology Develop- ment Fund; [WJ2019Q014]; [CXPJJH1200002-2020040]

基金资助正文：Acknowledgements This research was funded and supported by the Health Commission of Hubei Province scientific research project (no. WJ2019Q014) and Hubei Chen Xiaoping science and Technology Develop- ment Fund (no. CXPJJH1200002-2020040) .