A Novel Signal-On Electrochemiluminescence Immunosensor for the Detection of NSCLC Antigen Biomarker Based on New Co-Reaction Accelerators
作者全名："Guo, Lei; Mu, Zhaode; Qing, Min; Zhou, Jing; Li, Huizhen; Wang, Lulu; Zhong, Muyue; Bai, Lijuan"
作者地址："[Guo, Lei; Qing, Min; Zhou, Jing; Bai, Lijuan] Chongqing Med Univ, Coll Pharm, Chongqing Res Ctr Pharmaceut Engn, Chongqing 400016, Peoples R China; [Mu, Zhaode; Li, Huizhen; Wang, Lulu; Zhong, Muyue] Chongqing Med Univ, Coll Pharm, Res Ctr Pharmacodynam Evaluat Engn Technol Chongqi, Chongqing 400016, Peoples R China"
通信作者："Bai, LJ (通讯作者)，Chongqing Med Univ, Coll Pharm, Chongqing Res Ctr Pharmaceut Engn, Chongqing 400016, Peoples R China."
来源：ADVANCED HEALTHCARE MATERIALS
ESI学科分类：MATERIALS SCIENCE
WOS号：WOS:000899643000001
JCR分区：Q1
影响因子：10
年份：2023
卷号：
期号：
开始页：
结束页：
文献类型：Article; Early Access
关键词：bimetallic organic frameworks; electrochemiluminescence; multiple signal amplification; NiMn layer double hydroxides; non-small cell lung cancer
摘要："Non-small cell lung cancer (NSCLC) is the most common type of lung cancer with substantial morbidity and mortality. Herein, a new signal-on electrochemiluminescence (ECL) immunosensor based on multiple amplification strategies is constructed for ultrasensitive detection of cytokeratin 19 fragment antigen 21-1 (CYFRA21-1) biomarker related to NSCLC. Polyethyleneimine (PEI) functionalized MXene is decorated with NiMn layer double hydroxide (NiMn LDH) to form MXene-PEI-NiMn LDH composite. Specially, the La-MOF@ZIF-67 bimetallic organic framework (named as LZBM) and MXene-PEI-NiMn LDH both served as coreaction accelerators to improve the ECL emission of the luminol-H2O2 system. To be specific, Au nanoparticles (AuNPs) coated MXene-PEI-NiMn LDH is applied to immobilize primary CYFRA21-1 antibody (Ab(1)), while AuNPs decorated LZBM was used for the loading of luminol and secondary CYFRA21-1 antibody (Ab(2)) to form tracer label. Therefore, the ECL signal of the sandwich-type immunosensor is significantly enhanced due to the high loading capability for luminol and the synergistic catalytic ability for the decomposition of H2O2 into reactive oxygen species (ROS). Under the optimal experimental conditions, the ECL immunosensor exhibited good analytical performances for CYFRA21-1 detection with a wide linear range (100 fg mL(-1)-100 ng mL(-1)) and a low limit of detection (85.20 fg mL(-1)), providing a promising method for early diagnosis of NSCLC."
基金机构："National Natural Science Foundation of China [82072378, 81601856]; Chongqing Talents: Exceptional Young Talents Project, China [CQYC202005015, cstc2021ycjh-bgzxm0328]; Ba Yu Scholar Program, China [YS2019020]; Program for Youth Innovation in Future Medicine, Chongqing Medical University, China [W0067]; Funds for High-Level Young Science and Technology Talent Cultivation Plan in Chongqing Medical University, China; Discipline Talent Training Program of College of Pharmacy in Chongqing Medical University, China [YXY2019XSGG4, YXY2021BSH04]; Postdoctoral Science Foundation of Chongqing Natural Science Foundation, China [CSTB2022NSCQ-BHX0006]"
基金资助正文："L.G. and Z.M. contributed equally to this work. This work is supported by the National Natural Science Foundation of China (82072378, 81601856), Chongqing Talents: Exceptional Young Talents Project, China (CQYC202005015, cstc2021ycjh-bgzxm0328), Ba Yu Scholar Program, China (YS2019020), Program for Youth Innovation in Future Medicine, Chongqing Medical University, China (W0067), Funds for High-Level Young Science and Technology Talent Cultivation Plan in Chongqing Medical University, China (2019), Discipline Talent Training Program of College of Pharmacy in Chongqing Medical University, China (YXY2019XSGG4, YXY2021BSH04) and Postdoctoral Science Foundation of Chongqing Natural Science Foundation, China (CSTB2022NSCQ-BHX0006)."
