LncRNA 148400 Promotes the Apoptosis of Renal Tubular Epithelial Cells in Ischemic AKI by Targeting the miR-10b-3p/GRK4 Axis

作者全名："Li, Xingjin; Wu, Zhifen; Yang, Jurong; Zhang, Dongshan"

作者地址："[Li, Xingjin; Zhang, Dongshan] Cent South Univ, Xiangya Hosp 2, Dept Emergency Med, Changsha 410011, Peoples R China; [Li, Xingjin; Zhang, Dongshan] Cent South Univ, Xiangya Hosp 2, Emergency Med & Difficult Dis Inst, Changsha 410011, Peoples R China; [Li, Xingjin; Zhang, Dongshan] Cent South Univ, Xiangya Hosp 2, Dept Nephrol, Changsha 410011, Peoples R China; [Wu, Zhifen; Yang, Jurong] Chongqing Med Univ, Dept Nephrol, Affiliated Hosp 3, Chongqing 401120, Peoples R China"

通信作者："Zhang, DS (通讯作者)，Cent South Univ, Xiangya Hosp 2, Dept Emergency Med, Changsha 410011, Peoples R China.; Zhang, DS (通讯作者)，Cent South Univ, Xiangya Hosp 2, Emergency Med & Difficult Dis Inst, Changsha 410011, Peoples R China.; Zhang, DS (通讯作者)，Cent South Univ, Xiangya Hosp 2, Dept Nephrol, Changsha 410011, Peoples R China.; Yang, JR (通讯作者)，Chongqing Med Univ, Dept Nephrol, Affiliated Hosp 3, Chongqing 401120, Peoples R China."

来源：CELLS

ESI学科分类：BIOLOGY & BIOCHEMISTRY

WOS号：WOS:000902296100001

JCR分区：Q2

影响因子：6

年份：2022

卷号：11

期号：24

开始页：　

结束页：　

文献类型：Article

关键词：AKI; lncRNA148400; miR-10b-3p; GRK4; apoptosis

摘要："Although recent studies have reported that long non-coding RNA (lncRNA) is involved in the development of ischemic acute kidney injury (AKI), the exact function and regulatory mechanism of lncRNAs in ischemic AKI remain largely unknown. Herein, we found that ischemic injury promoted the expression of lncRNA 148400 in mouse proximal tubule-derived cell line (BUMPT) and C57BL/6J mice. Furthermore, the lncRNA148400 mediates ischemic injury-induced apoptosis of BUMPT cells. Mechanistically, lncRNA 148400 sponged miR-10b-3p to promote apoptosis via GRK4 upregulation. Finally, knockdown of lncRNA 148400 alleviated the I/R-induced deterioration of renal function, renal tubular injury, and cell apoptosis. In addition, cleaved caspase-3 is increased via targeting the miR-10b-3p/GRK4 axis. Collectively, these results showed that lncRNA 148400/miR-10b-3p/GRK4 axis mediated the development of ischemic AKI."

基金机构：National Natural Science Foundation of China [81870475]; Changsha Science and Technology Bureau project [kq2001039]; Key Project of Hunan provincial science and technology innovation [2020SK1014]; Department of Science and Technology of Hunan Province project of International Cooperation and Exchanges [2020WK2009]; Fundamental Research China Hunan Provincial Science and Technology Department [2021SK4004]; Chongqing Talent Program Project [cstc2021ycjh-bgzxm0090]

基金资助正文：The study was supported in part by a grant from National Natural Science Foundation of China (81870475). Changsha Science and Technology Bureau project (kq2001039). Key Project of Hunan provincial science and technology innovation (2020SK1014). Department of Science and Technology of Hunan Province project of International Cooperation and Exchanges (2020WK2009). Fundamental Research China Hunan Provincial Science and Technology Department (2021SK4004). The Chongqing Talent Program Project (cstc2021ycjh-bgzxm0090).