A Self-Assembling Peptide as a Model for Detection of Colorectal Cancer

作者全名："Wan, Yuan; Luo, Ruyue; Chen, Jialei; Luo, Xinyi; Liu, Guicen; Su, Di; Lu, Na; Liu, Qichen; Luo, Zhongli"

作者地址："[Wan, Yuan; Luo, Ruyue; Chen, Jialei; Luo, Xinyi; Liu, Guicen; Su, Di; Lu, Na; Luo, Zhongli] Chongqing Med Univ, Coll Basic Med Sci, Mol Med & Canc Res Ctr, Chongqing 400016, Peoples R China; [Luo, Ruyue] Northwestern Univ Feinberg, Dept Med, Sch Med, Chicago, IL 60611 USA; [Liu, Qichen] Chongqing Med Univ, Coll Pediat, Chongqing 400016, Peoples R China"

通信作者："Luo, ZL (通讯作者)，Chongqing Med Univ, Coll Basic Med Sci, Mol Med & Canc Res Ctr, Chongqing 400016, Peoples R China."

来源：GELS

ESI学科分类：　

WOS号：WOS:000902720800001

JCR分区：Q1

影响因子：4.6

年份：2022

卷号：8

期号：12

开始页：　

结束页：　

文献类型：Article

关键词：self-assembling peptide; colorectal cancer; accurate molecular detection; three-dimensional culture

摘要："Patient-derived organoid (PDO) models have been widely used in precision medicine. The inability to standardize organoid creation in pre-clinical models has become apparent. The common mouse-derived extracellular matrix can no longer meet the requirements for the establishment of PDO models. Therefore, in order to develop effective methods for 3D cultures of organoids, we designed a self-assembling peptide, namely DRF3, which can be self-assembled into ordered fibrous scaffold structures. Here, we used the co-assembly of self-assembling peptide (SAP) and collagen type I, fibronectin, and laminin (SAP-Matrix) to co-simulate the extracellular matrix, which significantly reduced the culture time of PDO, improved the culture efficiency, and increased the self-assembly ability of cells. Compared with the results from the 2D cell line, the PDO showed a more significant expression of cancer-related genes. During organoid self-assembly, the expression of cancer-related genes is increased. These findings provide a theoretical basis for the establishment of precision molecular modeling platforms in the future."

基金机构："Chengdu Sciobio Surgery Institute, China; National Natural Science Foundation of China [CSSI-JZ20211021-0002]; [31771101]"

基金资助正文："This program is support by Chengdu Sciobio Surgery Institute, China (Project Number: CSSI-JZ20211021-0002), Zhongli Luo was supported by the grant from the National Natural Science Foundation of China (Project Number: NSFC 31771101)."