m6A regulator-mediated methylation modification patterns and immune microenvironment infiltration characterization in osteoarthritis

作者全名："Hu, Shidong; Shen, Chen; Yao, Xudong; Zou, Yulong; Wang, Ting; Sun, Xianding; Nie, Mao"

作者地址："[Hu, Shidong; Shen, Chen; Yao, Xudong; Zou, Yulong; Wang, Ting; Sun, Xianding; Nie, Mao] Chongqing Med Univ, Affiliated Hosp 2, Ctr Joint Surg, Dept Orthoped Surg, 76 Linjiang Rd, Chongqing 400016, Peoples R China"

通信作者："Nie, M (通讯作者)，Chongqing Med Univ, Affiliated Hosp 2, Ctr Joint Surg, Dept Orthoped Surg, 76 Linjiang Rd, Chongqing 400016, Peoples R China."

来源：BMC MEDICAL GENOMICS

ESI学科分类：MOLECULAR BIOLOGY & GENETICS

WOS号：WOS:000906109000001

JCR分区：Q3

影响因子：2.7

年份：2022

卷号：15

期号：1

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结束页：　

文献类型：Article

关键词：RNA N6-methyladenosine; Osteoarthritis; Bioinformatic analysis; Subtype classification; Immune infiltration

摘要："Osteoarthritis (OA) is a common disease in orthopedics. RNA N6-methyladenosine (m6A) exerts an essential effect in a variety of biological processes in the eukaryotes. In this study, we determined the effect of m6A regulators in the OA along with performing the subtype classification. Differential analysis of OA and normal samples in the database of Gene Expression Omnibus identified 9 significantly differentially expressed m6A regulators. These regulators were monitored by a random forest algorithm so as to evaluate the risk of developing OA disease. On the basis of these 9 moderators, a nomogram was established. The results of decision curve analysis suggested that the patients could benefit from a nomogram model. The OA sample was classified as 2 m6A models through a consensus clustering algorithm in accordance with these 9 regulators. These 2 m6A patterns were then assessed with principal component analysis. We also determined the m6A scores for the 2 m6A patterns and their correlation with immune infiltration. The results indicated that type A had a higher m6A score than type B. Thus, we suggest that the m6A pattern may provide a new approach for diagnose and provide novel ideas for molecular targeted therapy of OA."

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