MiR-652-3p promotes malignancy and metastasis of cancer cells via inhibiting TNRC6A in hepatocellular carcinoma

作者全名："Chen, Wei; Ru, Junnan; Wu, Tong; Man, Da; Wu, Jingbang; Wu, Lijuan; Sun, Yujing; Yu, Hanxi; Li, Min; Zhang, Gangwei; Zhu, Xingxin; Tong, Rongliang; Xiao, Heng; Li, Yanhua; Yang, Beng"

作者地址："[Chen, Wei; Wu, Lijuan; Li, Min; Zhang, Gangwei; Li, Yanhua] Zhejiang Chinese Med Univ, Gen Practice Dept, Affiliated Hosp 2, Hangzhou, Zhejiang, Peoples R China; [Ru, Junnan; Man, Da; Wu, Jingbang; Zhu, Xingxin; Tong, Rongliang; Yang, Beng] Zhejiang Univ, Sch Med, Affiliated Hosp 1, Div Hepatobiliary & Pancreat Surg,Dept Surg, Hangzhou, Zhejiang, Peoples R China; [Sun, Yujing] Zhejiang Univ, Affiliated Hosp 1, Gen Practice Dept, Sch Med, Hangzhou, Zhejiang, Peoples R China; [Yu, Hanxi] Zhejiang Univ, Sch Med, Affiliated Hosp 1, Hlth Management Ctr, Hangzhou, Zhejiang, Peoples R China; [Xiao, Heng] Chongqing Med Univ, Dept Hepatobiliary Surg, Affiliated Hosp 1, Chongqing, Peoples R China; [Wu, Tong] Zhejiang Univ, Sch Med, Hangzhou, Zhejiang, Peoples R China"

通信作者："Li, YH (通讯作者)，Zhejiang Chinese Med Univ, Gen Practice Dept, Affiliated Hosp 2, Hangzhou, Zhejiang, Peoples R China.; Yang, B (通讯作者)，Zhejiang Univ, Sch Med, Affiliated Hosp 1, Div Hepatobiliary & Pancreat Surg,Dept Surg, Hangzhou, Zhejiang, Peoples R China."

来源：BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

ESI学科分类：BIOLOGY & BIOCHEMISTRY

WOS号：WOS:000927575800001

JCR分区：Q3

影响因子：2.5

年份：2023

卷号：640

期号：　

开始页：1

结束页：11

文献类型：Article

关键词：Hepatocellular carcinoma; Metastasis; miR-652-3p; TNRC6A

摘要："Background: Hepatocellular carcinoma (HCC) was one of the most prevalent life-threatening cancers. Metastasis is the leading cause of cancer-related death in HCC. MiRNAs play essential roles in cancer metastasis. Methods: Expression of miR-652-3p in HCC was assessed. Function experiments of miR-652-3p and trinucleotide repeat-containing gene 6A protein (TNRC6A) were performed both in vitro and in vivo. mRNA sequencing, PCR, and western blot were performed to verify the target genes and pathway of miR-652-3p. The lung metastasis and xenograft cancer model in nude mice was established to investigate the effects of the miR-652-3p and TRNC6A on tumor metastasis in vivo. The relationship between the expression of the miR-652-3p, TNRC6A and the prognosis of HCC patients was analyzed. Results: Upregulated miR-652-3p was found in the tumor tissues of HCC, especially in metastatic HCC patients. Overexpression of miR-652-3p promoted and knockdown of miR-652-3p suppressed HCC metastasis both in vitro and in vivo. MiR-652-3p promoted HCC metastasis via regulating the EMT pathway. TNRC6A was identified as a direct target of miR-652-3p, and the knockdown of TNRC6A restored repressed EMT and HCC metastasis caused by the inhibition of miR-652-3p. Clinical results revealed that high expression of miR-652-3p and low expression of TNRC6A were positively correlated to shortened overall survival and disease-free survival in HCC patients. Conclusions: MiR-652-3p promotes EMT and HCC metastasis by inhibiting TNRC6A expression in HCC. MiR-652-3p and TNRC6A may serve as potential biomarkers to predict prognosis in HCC patients with metastasis. (c) 2022 Elsevier Inc. All rights reserved."

基金机构：Medical Health Science and Technology Project of Zhejiang Province [2020KY675]; Natural Science Foundation of Zhejiang Province [LQ20H160037]

基金资助正文：This study was supported by a grant from the Medical Health Science and Technology Project of Zhejiang Province (No. 2020KY675) and the Natural Science Foundation of Zhejiang Province (LQ20H160037).