Lactate Mediates the Bone Anabolic Effect of High-Intensity Interval Training by Inducing Osteoblast Differentiation

作者全名："Zhu, Zhenglin; Chen, Yi; Zou, Jing; Gao, Shengqiang; Wu, Dandong; Li, Xuelun; Hu, Ning; Zhao, Jinzhong; Huang, Wei; Chen, Hong"

作者地址："[Zhu, Zhenglin; Chen, Yi; Zou, Jing; Gao, Shengqiang; Wu, Dandong; Li, Xuelun; Hu, Ning; Zhao, Jinzhong; Huang, Wei; Chen, Hong] Chongqing Med Univ, Affiliated Hosp 1, Chongqing, Peoples R China; [Zhu, Zhenglin; Zou, Jing; Gao, Shengqiang; Li, Xuelun; Hu, Ning; Huang, Wei; Chen, Hong] Chongqing Med Univ, Affiliated Hosp 1, Dept Orthoped, Chongqing, Peoples R China; [Zhu, Zhenglin; Zou, Jing; Gao, Shengqiang; Li, Xuelun; Hu, Ning; Huang, Wei; Chen, Hong] Chongqing Med Univ, Orthoped Lab, Chongqing, Peoples R China; [Chen, Yi] Chongqing Med Univ, Affiliated Hosp 2, Dept Rehabil, Chongqing, Peoples R China; [Wu, Dandong] Chongqing Med Univ, Affiliated Hosp 1, Dept Rehabil, Chongqing, Peoples R China; [Zhao, Jinzhong] Shanghai Jiao Tong Univ, Affiliated Sixth Peoples Hosp, Dept Sports Med, Shanghai, Peoples R China"

通信作者："Chen, H (通讯作者)，Chongqing Med Univ, Affiliated Hosp 1, Chongqing, Peoples R China.; Chen, H (通讯作者)，Chongqing Med Univ, Affiliated Hosp 1, Dept Orthoped, Chongqing, Peoples R China.; Chen, H (通讯作者)，Chongqing Med Univ, Orthoped Lab, Chongqing, Peoples R China."

来源：JOURNAL OF BONE AND JOINT SURGERY-AMERICAN VOLUME

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:000941639300008

JCR分区：Q1

影响因子：4.4

年份：2023

卷号：105

期号：5

开始页：369

结束页：379

文献类型：Article

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摘要："Background:High-intensity interval training (HIIT) reportedly improves bone metabolism and increases bone mineral density (BMD). The purpose of the present study was to investigate whether lactate mediates the beneficial effects of exercise on BMD, bone microarchitecture, and biomechanical properties in an established osteoporotic animal model. In addition, we hypothesized that lactate-induced bone augmentation is achieved through enhanced osteoblast differentiation and mineralization.Methods:A total of 50 female C57BL/6 mice were randomly allocated into 5 groups: the nonovariectomized group, the ovariectomized group (OVX), the HIIT group (OVX + HIIT), the HIIT with lactate transporter inhibition group (OVX + HIIT + INH), and the lactate subcutaneous injection group (OVX + LAC). After 7 weeks of intervention, bone mass, bone strength, and bone formation/resorption processes were evaluated via microcomputed tomography (micro-CT), biomechanical testing, histological analysis, and serum biochemical assays; in vitro studies were performed to explore the bone anabolic effect of lactate at the cellular level.Results:Micro-CT revealed significantly increased BMD in both the OVX + HIIT group (mean difference, 41.03 mg hydroxyapatite [HA]/cm(3) [95% CI, 2.51 to 79.54 mg HA/cm(3)]; p = 0.029) and the OVX + LAC group (mean difference, 40.40 mg HA/cm(3) [95% CI, 4.08 to 76.71 mg HA/cm(3)]; p = 0.031) compared with the OVX group. Biomechanical testing demonstrated significantly improved mechanical properties in those 2 groups. However, the beneficial effects of exercise on bone microstructure and biomechanics were largely abolished by blocking the lactate transporter. Notably, histological and biochemical results indicated that increased bone formation was responsible for the bone augmentation effects of HIIT and lactate. Cell culture studies showed a marked increase in the expression of osteoblastic markers with lactate treatment, which could be eliminated by blocking the lactate transporter.Conclusions:Lactate may have mediated the bone anabolic effect of HIIT in osteoporotic mice, which may have resulted from enhanced osteoblast differentiation and mineralization."

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