CDGSH iron sulfur domain 2 over-expression alleviates neuronal ferroptosis and brain injury by inhibiting lipid peroxidation via AKT/mTOR pathway following intracerebral hemorrhage in mice

作者全名："Li, Ruihao; Zhang, Xingyu; Gu, Lingui; Yuan, Ye; Luo, Xu; Shen, Weiwei; Xie, Zongyi"

作者地址："[Li, Ruihao; Zhang, Xingyu; Gu, Lingui; Yuan, Ye; Luo, Xu; Xie, Zongyi] Chongqing Med Univ, Affiliated Hosp 2, Dept Neurosurg, 76 Linjiang Rd, Chongqing 400010, Peoples R China; [Shen, Weiwei] Chongqing Med & Pharmaceut Coll, Affiliated Hosp 1, Dept Endocrinol, 301 Dashi Rd, Chongqing 400060, Peoples R China"

通信作者："Xie, ZY (通讯作者)，Chongqing Med Univ, Affiliated Hosp 2, Dept Neurosurg, 76 Linjiang Rd, Chongqing 400010, Peoples R China.; Shen, WW (通讯作者)，Chongqing Med & Pharmaceut Coll, Affiliated Hosp 1, Dept Endocrinol, 301 Dashi Rd, Chongqing 400060, Peoples R China."

来源：JOURNAL OF NEUROCHEMISTRY

ESI学科分类：NEUROSCIENCE & BEHAVIOR

WOS号：WOS:000946009800001

JCR分区：Q2

影响因子：4.2

年份：2023

卷号：165

期号：3

开始页：426

结束页：444

文献类型：Article

关键词：brain injury; CISD2; ferroptosis; intracerebral hemorrhage; lipid peroxidation

摘要："Ferroptosis has been implicated in the pathogenesis of secondary brain injury following intracerebral hemorrhage (ICH), and regulating this process is considered a potential therapy for alleviating further brain injury. A previous study showed that CDGSH iron sulfur domain 2 (CISD2) can inhibit ferroptosis in cancer. Thus, we investigated the effects of CISD2 on ferroptosis and the mechanisms underlying its neuroprotective role in mice after ICH. CISD2 expression markedly increased after ICH. CISD2 over-expression significantly decreased the number of Fluoro-Jade C-positive neurons and alleviated brain edema and neurobehavioral deficits at 24 h after ICH. In addition, CISD2 over-expression up-regulated the expression of p-AKT, p-mTOR, ferritin heavy chain 1, glutathione peroxidase 4, ferroportin, glutathione, and glutathione peroxidase activity, which are markers of ferroptosis. Additionally, CISD2 over-expression down-regulated the levels of malonaldehyde, iron content, acyl-CoA synthetase long-chain family member 4, transferrin receptor 1, and cyclooxygenase-2 at 24 h after ICH. It also alleviated mitochondrial shrinkage and decreased the density of the mitochondrial membrane. Furthermore, CISD2 over-expression increased the number of GPX4-positive neurons following ICH induction. Conversely, knockdown of CISD2 aggravated neurobehavioral deficits, brain edema, and neuronal ferroptosis. Mechanistically, MK2206, an AKT inhibitor, suppressed p-AKT and p-mTOR and reversed the effects of CISD2 over-expression on markers of neuronal ferroptosis and acute neurological outcome. Taken together, CISD2 over-expression alleviated neuronal ferroptosis and improved neurological performance, which may be mediated through the AKT/mTOR pathway after ICH. Thus, CISD2 may be a potential target to mitigate brain injury via the anti-ferroptosis effect after ICH."

基金机构：Chongqing Science and Health Joint Medical Research Project [2020GDRC006]; Kuanren Talents Program of the Second Affiliated Hospital of Chongqing Medical University [201959]; Venture & Innovation Support Program for Chongqing Overseas Returnees [CX2019156]

基金资助正文："Chongqing Science and Health Joint Medical Research Project, Grant/Award Number: 2020GDRC006; Kuanren Talents Program of the Second Affiliated Hospital of Chongqing Medical University, Grant/Award Number: 201959; Venture & Innovation Support Program for Chongqing Overseas Returnees, Grant/Award Number: CX2019156"