Specific capture and determination of glycoprotein using a hybrid epitopes and monomers-mediated molecular-imprinted polymer enzyme-free electrochemical biosensor

作者全名："He, Jia-Yuan; Xu, Hui-Xian; Li, Qin; Zheng, Qin-Yue; Wang, Chong-Zhi; Zhou, Lian-Di; Luo, Ling; Zhang, Qi-Hui; Yuan, Chun-Su"

作者地址："[He, Jia-Yuan; Xu, Hui-Xian; Li, Qin; Zhang, Qi-Hui] Chongqing Univ, Sch Chem & Chem Engn, Chongqing 400044, Peoples R China; [Zhou, Lian-Di] Chongqing Med Univ, Basic Med Coll, Chongqing 400016, Peoples R China; [Luo, Ling] Chongqing Univ, Canc Hosp, Chongqing Canc Inst, Chongqing 400030, Peoples R China; [Zheng, Qin-Yue] Chongqing Inst Food & Drug Control, Chongqing 401121, Peoples R China; [Wang, Chong-Zhi; Zhang, Qi-Hui; Yuan, Chun-Su] Univ Chicago, Tang Ctr Herbal Med Res, Chicago, IL 60637 USA; [Wang, Chong-Zhi; Zhang, Qi-Hui; Yuan, Chun-Su] Univ Chicago, Dept Anesthesia & Crit Care, Chicago, IL 60637 USA"

通信作者："Zhang, QH (通讯作者)，Chongqing Univ, Sch Chem & Chem Engn, Chongqing 400044, Peoples R China.; Zhou, LD (通讯作者)，Chongqing Med Univ, Basic Med Coll, Chongqing 400016, Peoples R China.; Luo, L (通讯作者)，Chongqing Univ, Canc Hosp, Chongqing Canc Inst, Chongqing 400030, Peoples R China.; Zhang, QH (通讯作者)，Univ Chicago, Tang Ctr Herbal Med Res, Chicago, IL 60637 USA.; Zhang, QH (通讯作者)，Univ Chicago, Dept Anesthesia & Crit Care, Chicago, IL 60637 USA."

来源：MICROCHIMICA ACTA

ESI学科分类：CHEMISTRY

WOS号：WOS:000946237000003

JCR分区：Q1

影响因子：5.3

年份：2023

卷号：190

期号：4

开始页：　

结束页：　

文献类型：Article

关键词：Electrochemical sensor; Surface-modified glassy-carbon electrode; Differential-pulse voltammetry; Epitope molecular imprinting; Transferrin; Glycoprotein; Multi-walled carbon nanotubes

摘要："A novel molecular-imprinted polymer (MIP)-based enzyme-free biosensor was created for the selective detection of glycoprotein transferrin (Trf). For this purpose, MIP-based biosensor for Trf was prepared by electrochemical co-polymerization of novel hybrid monomers 3-aminophenylboronic acid (M-APBA) and pyrrole on a glassy carbon electrode (GCE) modified with carboxylated multi-walled carbon nanotubes (cMWCNTs). Hybrid epitopes of Trf (C-terminal fragment and glycan) have been selected as templates. The produced sensor exhibited great selective recognition ability toward Trf under optimal preparation conditions, offering good analytical range (0.125-1.25 mu M) with a detection limit of 0.024 mu M. The proposed hybrid epitope in combination with hybrid monomer-mediated imprinting strategy was successfully applied to detect Trf in spiked human serum samples, with recoveries and relative standard deviations ranging from 94.7 to 106.0% and 2.64 to 5.32%, respectively. This study provided a reliable protocol for preparing hybrid epitopes and monomers-mediated MIP for the synergistic and effective determination of glycoprotein in complicated biological samples."

基金机构："National Natural Science Foundation of China [81973451]; Fundamental and Frontier Research Fund of Chongqing [cstc2018jcyjAX0661, cstc2019jsyj-yzysbAX0020]; Graduate Research and Innovation Project of Chongqing, 2022 [CYB22041]; Science and Technology Research Program of Chongqing Municipal Education Commission [KJZD-K201800103]; Fundamental Research Funds for the Central Universities [2019CDYGYB027, 2022CDJXY-003]; Graduate Research and Innovation Foundation of Chongqing [CYS18033]; Venture & Innovation Support Program for Chongqing Overseas Returnees; Tang Foundations"

基金资助正文："This work was financially supported by National Natural Science Foundation of China (No.81973451), Fundamental and Frontier Research Fund of Chongqing (No. cstc2018jcyjAX0661, No.cstc2019jsyj-yzysbAX0020), Graduate Research and Innovation Project of Chongqing, 2022 (CYB22041), the Science and Technology Research Program of Chongqing Municipal Education Commission (No. KJZD-K201800103), Fundamental Research Funds for the Central Universities (No. 2019CDYGYB027, 2022CDJXY-003), Graduate Research and Innovation Foundation of Chongqing (No. CYS18033), Venture & Innovation Support Program for Chongqing Overseas Returnees, and Tang Foundations."