Nanokit coupled electrospray ionization mass spectrometry for analysis of angiotensin converting enzyme 2 activity in single living cell

作者全名："Chang, Xinqi; Wang, Nina; Jiang, Depeng; Chen, Hong-Yuan; Jiang, Dechen"

作者地址："[Chang, Xinqi; Wang, Nina; Chen, Hong-Yuan; Jiang, Dechen] Nanjing Univ, Sch Chem & Chem Engn, State Key Lab Analyt Chem Life Sci, Nanjing 210093, Peoples R China; [Jiang, Depeng] Chongqing Med Univ, Affiliated Hosp 2, Dept Resp Med, Chongqing 400010, Peoples R China"

通信作者："Jiang, DC (通讯作者)，Nanjing Univ, Sch Chem & Chem Engn, State Key Lab Analyt Chem Life Sci, Nanjing 210093, Peoples R China.; Jiang, DP (通讯作者)，Chongqing Med Univ, Affiliated Hosp 2, Dept Resp Med, Chongqing 400010, Peoples R China."

来源：CHINESE CHEMICAL LETTERS

ESI学科分类：CHEMISTRY

WOS号：WOS:000950227600001

JCR分区：Q1

影响因子：9.4

年份：2023

卷号：34

期号：3

开始页：　

结束页：　

文献类型：Article

关键词：Nanokit; Electrospray ionization mass spectrometry; Single cell analysis; The enzymatic activity; ACE2

摘要："Angiotensin-converting enzyme 2 (ACE2) is not only an enzyme but also a functional receptor on cell membrane for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Here, the activity of ACE2 in single living cell is firstly determined using a nanokit coupled electrospray ionization mass spectrometry (nanokit-ESI-MS). Upon the insertion of a micro-capillary into the living hACE2-CHO cell and the electrochemical sorting of the cytosol, the target ACE2 enzyme hydrolyses angiotensin II inside the capillary to generate angiotensin 1-7. After the electrospray of the mixture at the tip of the capillary, the product is differentiated from the substrate in molecular weight to achieve the detection of ACE2 activity in single cells. The further measurement illustrates that the inflammatory state of cells does not lead to the significant change of ACE2 catalytic activity, which elucidates the relationship between intracellular ACE2 activity and inflammation at single cell level. The established strategy will provide a specific analytical method for further studying the role of ACE2 in the process of virus infection, and extend the application of nanokit based single cell analysis. (c) 2023 Published by Elsevier B.V. on behalf of Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences."

基金机构："Ministry of Science and Technology of China [2017YFA0700500]; National Natural Science Foundation of China [22025403, 21974060]; Kuanren Talents Program of the Second Affiliated Hospital of Chongqing Medical University; Young and Middle-aged Senior Medical Talents Studio of Chongqing"

基金资助正文："This work was supported by Ministry of Science and Technology of China (No. 2017YFA0700500) , the National Natural Science Foundation of China (Nos. 22025403 and 21974060) , Kuanren Tal-ents Program of the Second Affiliated Hospital of Chongqing Med-ical University, and Young and Middle-aged Senior Medical Talents Studio of Chongqing."