Effects of topical fluoride application on oral microbiota in young children with severe dental caries

作者全名："Yang, Zhengyan; Cai, Ting; Li, Yueheng; Jiang, Dan; Luo, Jun; Zhou, Zhi"

作者地址："[Yang, Zhengyan; Cai, Ting; Li, Yueheng; Luo, Jun; Zhou, Zhi] Chongqing Med Univ, Dept Prevent Dent, Stomatol Hosp, Chongqing, Peoples R China; [Yang, Zhengyan; Cai, Ting; Li, Yueheng; Jiang, Dan; Luo, Jun; Zhou, Zhi] Chongqing Key Lab Oral Biomed Engn Higher Educ, Dept Prevent Dent, Chongqing, Peoples R China; [Yang, Zhengyan; Li, Yueheng] Chongqing Key Lab Oral Dis & Biomed Sci, Chongqing, Peoples R China"

通信作者："Luo, J; Zhou, Z (通讯作者)，Chongqing Med Univ, Dept Prevent Dent, Stomatol Hosp, Chongqing, Peoples R China.; Luo, J; Zhou, Z (通讯作者)，Chongqing Key Lab Oral Biomed Engn Higher Educ, Dept Prevent Dent, Chongqing, Peoples R China."

来源：FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY

ESI学科分类：MICROBIOLOGY

WOS号：WOS:000953643000001

JCR分区：Q1

影响因子：4.6

年份：2023

卷号：13

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：microbial community; fluoride; dental caries; high-throughput sequencing; saliva

摘要："While the effect of fluoride on severe early childhood caries (S-ECC) is clear, knowledge of how it influences the oral microbiota and the consequential effects on oral health is limited. In this cohort study, we investigated the changes introduced in the oral ecosystem before and after using fluoride varnish in 54- to 66-month-old individuals (n=90: 18 children were sampled at 5 different time points). 16S rDNA was amplified from bacterial samples using polymerase chain reaction, and high-throughput sequencing was performed using Illumina MiSeq platforms. Many pronounced microbial changes were related to the effects of fluoride varnishing. The health-associated Bacteroides and Uncultured_bacterium_f_Enterobacteriaceae were enriched in the saliva microbiome following treatment with fluoride varnishing. Co-occurrence network analysis of the dominant genera showed that different groups clearly showed different bacterial correlations. The PICRUSt algorithm was used to predict the function of the microbial communities from saliva samples. The results showed that starch and sucrose metabolism was greater after fluoride use. BugBase was used to determine phenotypes present in microbial community samples. The results showed that Haemophilus and Neisseria (phylum Proteobacteria) was greater before fluoride use. We conclude that the changes in oral microbiology play a role in fluoride prevention of S-ECC."

基金机构：Natural Science Foundation Project of Chongqing [CSTC2019jcyj-msxmX0191]; Science Project of Chongqing Municipal Health Commission [2021MSXM31]; Program for Innovation Team Building at Institutions of Higher Education in Chongqing [CXTDG201602006]; First Batch of Key Disciplines On Public Health in Chongqing

基金资助正文："This study was supported by the Natural Science Foundation Project of Chongqing, No. CSTC2019jcyj-msxmX0191; Science Project of Chongqing Municipal Health Commission, No. 2021MSXM31, and the Program for Innovation Team Building at Institutions of Higher Education in Chongqing in 2016, No. CXTDG201602006; The First Batch of Key Disciplines On Public Health in Chongqing."