Electrochemical Biosensor for Detection of the CYP2C19*2 Allele Based on Exonuclease III

作者全名："Chen, Siling; Yu, Rongjun; Li, Ying; Wu, Jiangling; Qiu, Jingfu; Huang, Xinyi; Xue, Jianjiang"

作者地址："[Chen, Siling; Yu, Rongjun; Wu, Jiangling; Xue, Jianjiang] Chongqing Med Univ, Univ Town Hosp, Dept Clin Lab, Chongqing 401331, Peoples R China; [Li, Ying; Qiu, Jingfu] Chongqing Med Univ, Sch Publ Hlth & Management, Chongqing 400016, Peoples R China; [Huang, Xinyi] Guangxi Univ Chinese Med, Affiliated Hosp 1, Dept Clin Lab, Nanning 530023, Peoples R China"

通信作者："Xue, JJ (通讯作者)，Chongqing Med Univ, Univ Town Hosp, Dept Clin Lab, Chongqing 401331, Peoples R China.; Huang, XY (通讯作者)，Guangxi Univ Chinese Med, Affiliated Hosp 1, Dept Clin Lab, Nanning 530023, Peoples R China."

来源：MICROMACHINES

ESI学科分类：ENGINEERING

WOS号：WOS:000958887500001

JCR分区：Q2

影响因子：3

年份：2023

卷号：14

期号：3

开始页：　

结束页：　

文献类型：Article

关键词：clopidogrel; electrochemical biosensor; exonuclease III; calixarene

摘要："Currently, the therapeutic effect of clopidogrel differs considerably among individuals and is thought to be closely related to the genetic polymorphism of CYP2C19. The CYP2C19*2 gene can reduce the antiplatelet aggregation effect of clopidogrel, which increases the risk of major cardiovascular adverse events in patients. In this research, we report a new type of biosensor for the highly sensitive detection of the CYP2C19*2 gene based on exonuclease III assisted electric signal amplification and the use of calixarene to enrich electrical signal substances. Specifically, under the best conditions, the logarithmic concentrations of the analytes have a good linear relationship with the peak current in the range of 0.01 fM to 100 pM and the detection limit is 13.49 aM. The results have also shown that this method has good selectivity, high sensitivity, and stability, etc., and will provide a very promising application for the detection of the CYP2C19*2 gene and other biological molecules by replacing corresponding nucleic acid sequences."

基金机构："Science and Technology Bureau of Yuzhong District of Chongqing city, China [20200514]"

基金资助正文："This research was supported by Science and Technology Bureau of Yuzhong District of Chongqing city, China (Grant No. 20200514)."