Multifunctional Droplets Formed by Interfacially Self-Assembled Fluorinated Magnetic Nanoparticles for Biocompatible Single Cell Culture and Magnet-Driven Manipulation

作者全名："Liu, Jiahe; Lyu, Xiaoyan; Zhou, Ziwei; Yang, Lin; Zeng, Jie; Yang, Yao; Zhao, Zhenghuan; Chen, Rui; Tong, Xin; Li, Jiaqi; Liu, Hailan; Zou, Yuan"

作者地址："[Liu, Jiahe; Yang, Lin; Chen, Rui; Tong, Xin; Li, Jiaqi; Liu, Hailan; Zou, Yuan] Chongqing Med Univ, Coll Lab Med, Key Lab Clin Lab Diagnost, Minist Educ, Chongqing 400016, Peoples R China; [Lyu, Xiaoyan; Yang, Yao] Sichuan Univ, West China Hosp, Clin Inst Inflammat & Immunol, Frontiers Sci Ctr Dis, Chengdu 610041, Peoples R China; [Zhou, Ziwei] Chongqing Med Univ, Cent Lab Yongchuan Hosp, Chongqing 402160, Peoples R China; [Zeng, Jie; Zhao, Zhenghuan] Chongqing Med Univ, Coll Basic Med Sci, Chongqing 400016, Peoples R China"

通信作者："Zou, Y (通讯作者)，Chongqing Med Univ, Coll Lab Med, Key Lab Clin Lab Diagnost, Minist Educ, Chongqing 400016, Peoples R China."

来源：ACS APPLIED MATERIALS & INTERFACES

ESI学科分类：MATERIALS SCIENCE

WOS号：WOS:000959670300001

JCR分区：Q1

影响因子：8.3

年份：2023

卷号：15

期号：13

开始页：17324

结束页：17334

文献类型：Article

关键词：multifunctional droplets; amphiphilic magnetic nanoparticles; biocompatibility; non-leakage; magnetic manipulation

摘要："The ability to encapsulate and manipulate droplets with a picoliter volume of samples and reagents shows great potential for practical applications in chemistry, biology, and materials science. Magnetic control is a promising approach for droplet manipulation due to its ability for wireless control and its ease of implementation. However, it is challenged by the poor biocompatibility of magnetic materials in aqueous droplets. Moreover, current droplet technology is problematic because of the molecule leakage between droplets. In the paper, we propose multifunctional droplets with the surface coated by a layer of fluorinated magnetic nanoparticles for magnetically actuated droplet manipulation. Multifunctional droplets show excellent biocompatibility for cell culture, nonleakage of molecules, and high response to a magnetic field. We developed a strategy of coating the F-MNP@SiO2 on the outer surface of droplets instead of adding magnetic material into droplets to enable droplets with a highly magnetic response. The encapsulated bacteria and cells in droplets did not need to directly contact with the magnetic materials at the outer surface, showing high biocompatibility with living cells. These droplets can be precisely manipulated based on magnet distance, the time duration of the magnetic field, the droplet size, and the MNP composition, which well match with theoretical analysis. The precise magnetically actuated droplet manipulation shows great potential for accurate and sensitive droplet-based bioassays like single cell analysis."

基金机构："Natural Science Foundation of China [81871567]; Natural Science Foundation of Chongqing [2022NSCQ-MSX0784]; Project of Through-Train to Doctor of Chongqing [CSTB2022BSXM-JCX0035]; Science and Technology Research Program of Chongqing Municipal Education Commission [KJQN202100425]; Intelligent Medicine Research Project of Chongqing Medical University [ZHYX202111]; CQMU Program for Youth Innovation in Future Medicine [W0149]; Special Key Project of Technology Innovation and Application Development of Chongqing, China [cstc2019jscx-dxwtBX0032]; Chongqing Medical University"

基金资助正文："This work was supported by the Natural Science Foundation of China (81871567) , the Natural Science Foundation of Chongqing (2022NSCQ-MSX0784) , the Project of Through-Train to Doctor of Chongqing (CSTB2022BSXM-JCX0035) , the Science and Technology Research Program of Chongqing Municipal Education Commission (KJQN202100425) , the Intelligent Medicine Research Project of Chongqing Medical University (ZHYX202111) , CQMU Program for Youth Innovation in Future Medicine (W0149) , Special Key Project of Technology Innovation and Application Development of Chongqing, China (cstc2019jscx-dxwtBX0032) and start-up funding from Chongqing Medical University."