Intelligent recognition of CTCs from gallbladder cancer by ultrasensitive electrochemical cytosensor and diagnosis of chemotherapeutic resistance

作者全名："Zhang, Xiuzhen; Li, Lu; Zhang, Mi; Zhang, La; Liu, Shanshan; Guo, Jiao; Jiang, Ning; Peng, Qiling; Wang, Jianwei; Ding, Shijia"

作者地址："[Zhang, Xiuzhen; Li, Lu; Guo, Jiao; Peng, Qiling; Wang, Jianwei] Chongqing Med Univ, Sch Basic Med Sci, Chongqing 400016, Peoples R China; [Zhang, Mi] Chongqing Med Univ, Childrens Hosp, Dept Neurosurg, Chongqing 400016, Peoples R China; [Zhang, La; Liu, Shanshan] Chongqing Med Univ, Dept Hepatobiliary Surg, Chongqing 400016, Peoples R China; [Jiang, Ning] Chongqing Med Univ, Dept Pathol, Chongqing 400016, Peoples R China; [Jiang, Ning] Chongqing Med Univ, Mol Med Diagnost & Testing Ctr, Chongqing 400016, Peoples R China; [Jiang, Ning] Chongqing Med Univ, Affiliated Hosp 1, Dept Pathol, Chongqing 400016, Peoples R China; [Ding, Shijia] Chongqing Med Univ, Coll Lab Med, Key Lab Clin Lab Diagnost, Minist Educ, Chongqing 400016, Peoples R China"

通信作者："Peng, QL; Wang, JW (通讯作者)，Chongqing Med Univ, Sch Basic Med Sci, Chongqing 400016, Peoples R China.; Jiang, N (通讯作者)，Chongqing Med Univ, Dept Pathol, Chongqing 400016, Peoples R China."

来源：BIOSENSORS & BIOELECTRONICS

ESI学科分类：CHEMISTRY

WOS号：WOS:000961765500001

JCR分区：Q1

影响因子：10.7

年份：2023

卷号：228

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Gallbladder carcinoma; Chemoresistance; Circulating tumor cells; Cytosensor; Electrochemical probes

摘要："Gallbladder carcinoma (GBC) is one of most aggressive and lethal malignancies. Early diagnosis of GBC is crucial for determining appropriate treatment and improving chances of cure. Chemotherapy represents the main therapeutic regimen for unresectable GBC patients to inhibit tumor growth & metastasis. But, chemoresistance is the major cause of GBC recurrence. Thus, there is an urgent need to explore potentially non-invasive and point-of-care approaches to screen GBC and monitor their chemoresistance. Herein, we established an electrochemical cytosensor to specifically detect circulating tumor cells (CTCs) and their chemoresistance. Trilayer of CdSe/ZnS quantum dots (QDs) were cladded upon SiO2 nanoparticles (NPs), forming Tri-QDs/PEI@SiO2 electrochemical probes. Upon conjugation of anti-ENPP1, the electrochemical probes were able to specifically label captured CTCs from GBC. The detection of CTCs and chemoresistance were realized by square wave anodic stripping voltammetric (SWASV) responses to anodic stripping current of Cd 2+ ion when cadmium in electrochemical probes was dissolved and eventually electrodeposited on bismuth film-modified glassy carbon electrode (BFE). Taking use of this cytosensor, one ensured the screening of GBC and limit of detection for CTCs approaches to similar to 10 cells/mL. Furthermore, by monitoring phenotypic changes of CTCs after drug treatment, the diagnosis of chemoresistance was achieved by our cytosensor."

基金机构："National Natural Science Foundation of China [81972023]; Natural Science Foundation of Chongqing City [CSTC2020jcyj-msxmX0144, CSTB2022NSCQ-MSX0054, CSTC2021jcyj-msxm0172]; Science and Technology Research Program of Chongqing Education Commission of China [KJQN201900425, KJQN202000401]; Cre- ative Research Group of CQ University [CXQT21017]"

基金资助正文："This work was supported by the National Natural Science Foundation of China (Grant No. 81972023) , the Natural Science Foundation of Chongqing City (Grant No. CSTC2020jcyj-msxmX0144, CSTB2022NSCQ-MSX0054 and CSTC2021jcyj-msxm0172) , the Science and Technology Research Program of Chongqing Education Commission of China (Grant No. KJQN201900425 and KJQN202000401) , and Cre- ative Research Group of CQ University (Grant No. CXQT21017) ."