Identification of a novel plasma metabolite panel as diagnostic biomarker for hepatocellular carcinoma

作者全名："Liu, Zhiying; Liu, Hongtao; Chen, Zhiji; Deng, Chao; Zhou, Li; Chen, Siyuan; Kang, Juan; Chen, Yao; He, Song; Zhou, Zhihang"

作者地址："[Liu, Zhiying; Liu, Hongtao; Chen, Zhiji; Deng, Chao; Zhou, Li; Chen, Siyuan; He, Song; Zhou, Zhihang] Chongqing Med Univ, Dept Gastroenterol, Affiliated Hosp 2, Chongqing 400010, Peoples R China; [Kang, Juan] Chongqing Med Univ, Dept Infect Dis, Affiliated Hosp 2, Chongqing 400010, Peoples R China; [Chen, Yao] Chongqing Med Univ, Phys Examinat Ctr, Affiliated Hosp 2, Chongqing 400010, Peoples R China"

通信作者："He, S; Zhou, ZH (通讯作者)，Chongqing Med Univ, Dept Gastroenterol, Affiliated Hosp 2, Chongqing 400010, Peoples R China."

来源：CLINICA CHIMICA ACTA

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:000963858000001

JCR分区：Q2

影响因子：3.2

年份：2023

卷号：543

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Hepatocellular carcinoma; Cirrhosis; Metabolic biomarkers

摘要："Background and aims: Metabolic reprogramming is one of the hallmarks of cancer. Hepatocellular carcinoma (HCC) is one of the most lethal malignancy camcer, but the early diagnosis of HCC remains difficult. In this study, we searched for potential plasma metabolite biomarkers of HCC. Methods: A total of plasma samples of 104 HCC, 76 cirrhosis and 10 healthy subjects were assessed and validated through Gas chromatography-Mass spectrometry. Receiver-operating characteristic curves (ROC) combined with multivariate statistical analyses were used to assess the diagnostic performance of metabolites and combinations. Results: 10 metabolites in screening cohort were significantly changed in the plasma of HCC patients. Multi-variate logistic regression analysis of candidate metabolites in validation cohort showed that N-formylglycine, oxoglutaric acid, citrulline and heptaethylene glycol could distinguish HCC from cirrhosis. The combination of these four metabolites showed a better performance than AFP with the Area Under the Curve (AUC), sensitivity, specificity as 0.940, 84.00%, 97.56%, respectively. In further, the panel of N-formylglycine, heptaethylene glycol and citrulline can more effectively discriminate early stage HCC from cirrhosis than AFP (AUC: 0.835 vs. 0.634). Finally, heptaethylene glycol could significantly inhibit the proliferation, migration and invasion of HCC cells in vitro. Conclusion: The combination of plasma N-formylglycine, oxoglutaric acid, citrulline, and heptaethylene glycol can be an efficient novel diagnostic biomarker for HCC."

基金机构："National Natural Science Fund of China [81972285]; Natural Science Foundation of Chongqing (Chongqing, China) [CSTB2022NSCQ-MSX1038, 2022NSCQ-MAX0312, 2022NSCQ-MSX1069]; Senior Medi-cal Talents Program of Chongqing for Young and Middle-aged and Kuanren Talents Program of the Second Affiliated Hospital of Chongqing Medical University [13-002-011, 13-004-009]"

基金资助正文："Funding This work and publication costs were supported by the National Natural Science Fund of China (No. 81972285) ; the Natural Science Foundation of Chongqing (Chongqing, China, CSTB2022NSCQ-MSX1038, 2022NSCQ-MAX0312, 2022NSCQ-MSX1069) ; Senior Medi-cal Talents Program of Chongqing for Young and Middle-aged and Kuanren Talents Program of the Second Affiliated Hospital of Chongqing Medical University (13-002-011, 13-004-009) ."