A sandwich-type electrochemical immunosensor for sensitive detection of HER2 based on dual signal amplification of La-MOF-PbO2 and PEI-MoS2NFs composites

作者全名："Xia, Qin; Mu, Zhaode; Qing, Min; Zhou, Jing; Bai, Lijuan"

作者地址："[Xia, Qin; Zhou, Jing; Bai, Lijuan] Chongqing Med Univ, Coll Pharm, Chongqing Res Ctr Pharmaceut Engn, Chongqing 400016, Peoples R China; [Mu, Zhaode; Qing, Min] Chongqing Med Univ, Coll Pharm, Res Ctr Pharmacodynam Evaluat Engn Technol Chongqi, Chongqing 400016, Peoples R China"

通信作者："Bai, LJ (通讯作者)，Chongqing Med Univ, Coll Pharm, Chongqing Res Ctr Pharmaceut Engn, Chongqing 400016, Peoples R China."

来源：BIOELECTROCHEMISTRY

ESI学科分类：BIOLOGY & BIOCHEMISTRY

WOS号：WOS:000967518300001

JCR分区：Q1

影响因子：4.8

年份：2023

卷号：152

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：MoS2 nanoflowers; La-MOF-PbO2; Human epidermal growth factor receptor 2; Immunosensor; Breast cancer

摘要："In recent decades, the incidence of breast cancer has increased year by year, posing a serious threat to human health and quality of life, and about 30% of breast cancer patients have human epidermal growth factor receptor 2 (HER2) overexpression. Therefore, HER2 has become an important biomarker and indicator for the clinical evaluation of breast cancer in diagnosis, prognosis and recurrence. In this work, polyethyleneimine function-alized MoS2 nanoflowers (PEI-MoS2NFs) with good electrical conductivity and abundant active binding sites were designed and employed as a sensing platform for immobilizing the primary antibody of HER2 (Ab1). In addition, a La-MOF-PbO2 composite with a large specific surface area and good conductivity was used to load lots of electroactive toluidine blue (TB) and the secondary antibody of HER2 (Ab2) via gold nanoparticles (AuNPs) as the linker. Thus, the constructed sandwich-type electrochemical immunosensor was applied for sensitive detection of HER2, which showed a wide linear range from 100 fg mL-1 to 10 mu g mL-1 with a lower limit of detection of 15.64 fg mL-1. Therefore, the resulting immunosensor in this study would have a potential appli-cation in clinical bioanalysis."

基金机构："National Natural Science Foundation of China, China [82072378, 81601856]; Chongqing Talents: Exceptional Young Talents Project, China [CQYC202005015, cstc2021ycjh-bgzxm0328]; Ba Yu Scholar Program, China [YS2019020]; Special Funding for Postdoctoral Research Project of Chongqing, China [2021XM2045]; Natural Science Foundation of Chongqing, China [CSTB2022NSCQ-BHX0006]; Discipline Talent Training Program of College of Pharmacy in Chongqing Medical University, China [YXY2019XSGG4, YXY2021BSH04]; Funds for Young Science and Technology Talent Cultivation Plan of Chongqing City, China [cstc2014kjrc-qnrc00004]"

基金资助正文："This work is supported by National Natural Science Foundation of China, China (82072378, 81601856) , Chongqing Talents: Exceptional Young Talents Project, China (CQYC202005015, cstc2021ycjh-bgzxm0328) , Ba Yu Scholar Program, China (YS2019020) , Special Funding for Postdoctoral Research Project of Chongqing, China (2021XM2045) , Natural Science Foundation of Chongqing, China (CSTB2022NSCQ-BHX0006) , Discipline Talent Training Program of College of Pharmacy in Chongqing Medical University, China (YXY2019XSGG4, YXY2021BSH04) and Funds for Young Science and Technology Talent Cultivation Plan of Chongqing City, China (cstc2014kjrc-qnrc00004) ."