Integrative Analysis of Single-Cell and Bulk Sequencing Data Depicting the Expression and Function of P2ry12 in Microglia Post Ischemia-Reperfusion Injury

作者全名："Wang, Chenglong; Peng, Li; Wang, Yuan; Xue, Ying; Chen, Tianyi; Ji, Yanyan; Li, Yishan; Zhao, Yong; Yu, Shanshan"

作者地址："[Wang, Chenglong; Peng, Li; Wang, Yuan; Xue, Ying; Chen, Tianyi; Ji, Yanyan; Li, Yishan; Zhao, Yong; Yu, Shanshan] Chongqing Med Univ, Coll Basic Med, Dept Pathol, Chongqing 400016, Peoples R China; [Wang, Chenglong; Peng, Li; Wang, Yuan; Xue, Ying; Chen, Tianyi; Ji, Yanyan; Li, Yishan; Zhao, Yong; Yu, Shanshan] Chongqing Med Univ, Mol Med Diagnost & Testing Ctr, Chongqing 400016, Peoples R China; [Wang, Chenglong; Peng, Li; Wang, Yuan; Xue, Ying; Chen, Tianyi; Ji, Yanyan; Li, Yishan; Zhao, Yong; Yu, Shanshan] Chongqing Med Univ, Affiliated Hosp 1, Dept Pathol, Chongqing 400016, Peoples R China"

通信作者："Zhao, Y; Yu, SS (通讯作者)，Chongqing Med Univ, Coll Basic Med, Dept Pathol, Chongqing 400016, Peoples R China.; Zhao, Y; Yu, SS (通讯作者)，Chongqing Med Univ, Mol Med Diagnost & Testing Ctr, Chongqing 400016, Peoples R China.; Zhao, Y; Yu, SS (通讯作者)，Chongqing Med Univ, Affiliated Hosp 1, Dept Pathol, Chongqing 400016, Peoples R China."

来源：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

ESI学科分类：CHEMISTRY

WOS号：WOS:000968865900001

JCR分区：Q1

影响因子：4.9

年份：2023

卷号：24

期号：7

开始页：　

结束页：　

文献类型：Article

关键词：P2ry12; microglia; ischemia-reperfusion injury; bioinformatics

摘要："P2ry12 is a microglial marker gene. Recently, increasing evidence has demonstrated that its expression levels can vary in response to different CNS disorders and can affect microglial functions, such as polarization, plasticity, and migration. However, the expression and function of P2ry12 in microglia during ischemia-reperfusion injury (IRI) remain unclear. Here, we developed a computational method to obtain microglia-specific P2ry12 genes (MSPGs) using sequencing data associated with IRI. We evaluated the change in comprehensive expression levels of MSPGs during IRI and compared it to the expression of P2ry12 to determine similarity. Subsequently, the MSPGs were used to explore the P2ry12 functions in microglia through bioinformatics. Moreover, several animal experiments were also conducted to confirm the reliability of the results. The expression of P2ry12 was observed to decrease gradually within 24 h post injury. In response, microglia with reduced P2ry12 expression showed an increase in the expression of one receptor-encoding gene (Flt1) and three ligand-encoding genes (Nampt, Igf1, and Cxcl2). Furthermore, double-labeling immunofluorescence staining revealed that inhibition of P2ry12 blocked microglial migration towards vessels during IRI. Overall, we employ a combined computational and experimental approach to successfully explore P2ry12 expression and function in microglia during IRI."

基金机构：National Natural Science Foundation of Chongqing [cstc2020jcyj-msxmX0099]; National Natural Science Youth Foundation of China [82101376]

基金资助正文：This research was funded by the National Natural Science Foundation of Chongqing (No. cstc2020jcyj-msxmX0099) and the National Natural Science Youth Foundation of China (No. 82101376).