Untargeted metabolomics of saliva in caries-active and caries-free children in the mixed dentition

作者全名："Li, Yueheng; Yang, Zhengyan; Cai, Ting; Jiang, Dan; Luo, Jun; Zhou, Zhi"

作者地址："[Li, Yueheng; Yang, Zhengyan; Cai, Ting; Jiang, Dan; Luo, Jun; Zhou, Zhi] Chongqing Med Univ, Stomatol Hosp, Dept Prevent Dent, Chongqing, Peoples R China; [Li, Yueheng; Yang, Zhengyan; Cai, Ting; Jiang, Dan; Luo, Jun; Zhou, Zhi] Chongqing Med Univ, Coll Stomatol, Chongqing, Peoples R China; [Li, Yueheng; Yang, Zhengyan; Cai, Ting; Jiang, Dan; Luo, Jun; Zhou, Zhi] Chongqing Key Lab Oral Dis & Biomed Sci, Chongqing, Peoples R China; [Li, Yueheng; Yang, Zhengyan; Cai, Ting; Jiang, Dan; Luo, Jun; Zhou, Zhi] Chongqing Municipal Key Lab Oral Biomed Engn Highe, Chongqing, Peoples R China"

通信作者："Luo, J; Zhou, Z (通讯作者)，Chongqing Med Univ, Stomatol Hosp, Dept Prevent Dent, Chongqing, Peoples R China.; Luo, J; Zhou, Z (通讯作者)，Chongqing Med Univ, Coll Stomatol, Chongqing, Peoples R China.; Luo, J; Zhou, Z (通讯作者)，Chongqing Key Lab Oral Dis & Biomed Sci, Chongqing, Peoples R China.; Luo, J; Zhou, Z (通讯作者)，Chongqing Municipal Key Lab Oral Biomed Engn Highe, Chongqing, Peoples R China."

来源：FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY

ESI学科分类：MICROBIOLOGY

WOS号：WOS:000969384000001

JCR分区：Q1

影响因子：4.6

年份：2023

卷号：13

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：caries; saliva; untargeted metabolomics; children; mixed dentition

摘要："ObjectiveTo compare the differences in salivary metabolites between caries-active and caries-free children in the mixed dentition, and explore their correlation with caries status. MethodsThe study involved 20 children (aged 8-9 years) in the mixed dentition, including 10 caries-active (aged 8.6 +/- 0.49years) and 10 caries-free children(aged 8.5 +/- 0.5years), with a male/female ratio of 1:1. The saliva samples were collected from all children. Metabolite extraction, LC-MS/MS-based untargeted metabolomics, qualitative and semi-quantitative analysis and bioinformatics analysis were performed to identify differential metabolites between the two sample groups. The differential metabolites identified were further analyzed in an attempt to find their correlations with caries status. ResultsIn the positive ion mode, a total of 1606 molecular features were detected in the samples of the two groups, 189 of which were differential metabolites when comparing the caries-active group with the caries-free group, including 104 up-regulated and 85 down-regulated metabolites. In the negative ion mode, a total of 532 molecular features were detected in the samples of two groups, 70 of which were differential metabolites when comparing the caries-active group with the caries-free group, including 37 up-regulated and 33 down-regulated metabolites. In the positive ion mode, two of the top 5 up-regulated differential metabolites were found in and annotated to specific metabolic pathways, whereas in the negative ion mode, only one of the top 5 up-regulated differential metabolites was found in and annotated to specific metabolic pathways. In both the positive and negative ion modes, the top 5 down-regulated differential metabolites were both annotated to the metabolic pathways. KEGG pathway enrichment analysis of differential metabolites showed that histamine and arachidonic acid identified in the positive ion mode, as well as succinate and L-histidine identified in the negative ion mode were enriched in the top 3 significantly altered pathways. ConclusionThe enriched differential metabolites including histamine, L-histidine and succinate were correlated with the presence of dental caries, but their role in the caries process needs to be further investigated."

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