Molecular characteristics of multiple primary pulmonary nodules under a three-dimensional reconstruction model and relevant multi-omics analyses: a case report

作者全名："Luo, Zhilin; Xiao, Yajie; Luo, Chengwen; Zhang, Liping; Zhou, Runquan; Zhao, Zhikun; Sun, Chao; Wu, Dongfang; Wang, Tianhu"

作者地址："[Luo, Zhilin; Luo, Chengwen; Zhang, Liping; Zhou, Runquan; Wang, Tianhu] Chongqing Med Univ, Affiliated Hosp 3, Dept Thorac Surg, Chongqing, Peoples R China; [Xiao, Yajie; Zhao, Zhikun; Sun, Chao; Wu, Dongfang] YuceBio Technol Co Ltd, Dept Med, Shenzhen, Peoples R China"

通信作者："Wang, TH (通讯作者)，Chongqing Med Univ, Affiliated Hosp 3, Dept Thorac Surg, Chongqing, Peoples R China."

来源：FRONTIERS IN ONCOLOGY

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:000987135200001

JCR分区：Q2

影响因子：3.5

年份：2023

卷号：13

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：multiple primary lung cancer; multi-omics analyses; genomic profiling; tumor environment; three-dimensional (3D) reconstruction

摘要："Background: In addition to CT images and pathological features, many other molecular characteristics remain unknown about multiple primary lung cancer (MPLC) from intrapulmonary metastatic lung cancer. Case presentation: In this study, we reported a patient with an early-stage MPLC with both adenocarcinoma in situ (AIS) subtype and minimally invasive adenocarcinoma (MIA) subtype. The patient was diagnosed with more than 10 nodules and underwent precise surgery assisted by three-dimensional (3D) reconstruction at the left upper lung lobe. Whole-exome sequencing (WES) and multiple immunohistochemistry (mIHC) were performed to reveal the genomic profiling and tumor microenvironments of multiple nodules in this patient with MPLC. Based on 3D reconstruction location information, we found that the genomic and pathological results of adjacent lymph nodes were quite different. On the other hand, PD-L1 expression and the proportion of infiltrating lymphocytes in tumor microenvironments were all at a low status and did not vary in adjacent lymph nodes. Additionally, maximum diameter and tumor mutational burden levels were found to be significantly associated with CD8+ T cell proportion (p<0.05). Besides, CD163+ macrophages and CD4+ T cell proportion were higher in MIA nodules than in AIS nodules (p<0.05). This patient reached a recurrence-free survival of 39 months. Conclusion: Generally, in addition to CT imaging and pathological results, genomic profiling and tumor microenvironments may facilitate identifying the potential molecular mechanisms and clinical outcomes in patients with early-stage MPLC."

基金机构：　

基金资助正文：