"Mixed exposure effect of seminal metals on semen quality, mediation of total antioxidant capacity, and moderation of GSTM1/GSTT1 gene deletion in Chinese reproductive-aged men"

作者全名："Ren, Jingchao; Jin, Huidong; Zhang, Chen; Liu, Song; Han, Yufen; Xi, Jinyan; Cao, Jinhu; Lin, Fenglong; Zhang, Pingyang; Zhang, Fengquan; Zhang, Guang-hui; Yang, Huijun; Huang, Hefeng; Cao, Jia"

作者地址："[Ren, Jingchao] Chongqing Med Univ, Sch Publ Hlth & Management, Chongqing 400038, Peoples R China; [Jin, Huidong; Zhang, Guang-hui; Cao, Jia] Army Med Univ, Mil Med Univ 3, Coll Prevent Med, Dept Environm Hlth, Chongqing 400038, Peoples R China; [Zhang, Chen; Huang, Hefeng] Fudan Univ, Obstet & Gynecol Hosp, Inst Reprod & Dev, Shanghai 200032, Peoples R China; [Ren, Jingchao; Xi, Jinyan; Cao, Jinhu; Lin, Fenglong; Zhang, Pingyang; Zhang, Fengquan] Xinxiang Med Univ, Sch Publ Hlth, Henan Int Collaborat Lab Hlth Effects & Intervent, Xinxiang 453003, Peoples R China; [Liu, Song; Yang, Huijun] Maternal & Child Hlth Care Hosp Shandong Prov, Reprod Med Ctr, Jinan 250014, Peoples R China; [Han, Yufen] Puyang Matern & Child Care Ctr, 59 South Sect Kaizhou Rd, Puyang 457000, Peoples R China; [Zhang, Chen; Huang, Hefeng] Chinese Acad Med Sci No 2019RU056, Res Units Embryo Original Dis, Shanghai, Peoples R China; [Cao, Jia] Army Med Univ, Mil Med Univ 3, Coll Prevent Med, Key Lab Med Protect Electromagnet Radiat,Minist Ed, Chongqing 400038, Peoples R China"

通信作者："Huang, HF (通讯作者)，Fudan Univ, Obstet & Gynecol Hosp, Inst Reprod & Dev, Shanghai 200032, Peoples R China.; Yang, HJ (通讯作者)，Maternal & Child Hlth Care Hosp Shandong Prov, Reprod Med Ctr, Jinan 250014, Peoples R China.; Cao, J (通讯作者)，Army Med Univ, Mil Med Univ 3, Coll Prevent Med, Key Lab Med Protect Electromagnet Radiat,Minist Ed, Chongqing 400038, Peoples R China."

来源：ENVIRONMENTAL RESEARCH

ESI学科分类：ENVIRONMENT/ECOLOGY

WOS号：WOS:000988912100001

JCR分区：Q1

影响因子：7.7

年份：2023

卷号：229

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Metal; Sperm; GSTM1; GSTT1; Total antioxidant capacity; Bayesian kernel machine regression

摘要："Background: The effects of metal exposure on semen quality and the role of oxidative damage in this process remain unclear.Methods: We recruited 825 Chinese male volunteers, and 12 seminal metals (Mn, Cu, Zn, Se, Ni, Cd, Pb, Co, Ag, Ba, Tl, and Fe), the total antioxidant capacity (TAC), and reduced glutathione were measured. Semen parameters and GSTM1/GSTT1-null genotypes were also detected. Bayesian kernel machine regression (BKMR) was applied to evaluate the effect of the mixed exposure to metals on semen parameters. The mediation of TAC and moderation of GSTM1/GSTT1 deletion were analyzed.Results: Most seminal metal concentrations were correlated with each other. The BKMR models revealed a negative association between the semen volume and metal mixture, with Cd (cPIP = 0.60) and Mn (cPIP = 0.10) as the major contributors. Compared to fixing all scaled metals at their median value (50th percentiles), fixing the scaled metals at their 75th percentiles decreased the TAC by 2.17 units (95%CI:-2.60,-1.75). Mediation analysis indicated that Mn decreased the semen volume, with 27.82% of this association mediated by TAC. Both the BKMR and multi-linear models showed that seminal Ni was negatively correlated with sperm concentration, total sperm count, and progressive motility, which was modified by GSTM1/GSTT1. Furthermore, Ni and the total sperm count showed a negative association in GSTT1 and GSTM1 null males (beta[95%CI]: 0.328 [-0.521,-0.136]) but not in males with GSTT1 and/or GSTM1. Although Fe and the sperm concentration and total sperm count were positively correlated, they showed inverse ""U"" shapes in univariate analysis.Conclusion: Exposure to the 12 metals was negatively associated with semen volume, with Cd and Mn as the major contributors. TAC may mediate this process. GSTT1 and GSTM1 can modify the reduction in the total sperm count caused by seminal Ni exposure."

基金机构："National Key Research and Devel- opment Program [2021YFC2701601]; National Natural Science Foundation of China [82273663]; Youth project of National Natural Science Foundation of China [82003489, 82001571]; Open Fund Project of Guangdong Academy of Medical Sciences of China [YKY- KF202202]"

基金资助正文："This study was supported by the National Key Research and Devel- opment Program (2021YFC2701601) , the National Natural Science Foundation of China (82273663) , and the Youth project of National Natural Science Foundation of China (No: 82003489, 82001571) , Open Fund Project of Guangdong Academy of Medical Sciences of China (YKY- KF202202) ."