Point-of-care detection of Neisseria gonorrhoeae based on RPA-CRISPR/Cas12a

作者全名："Tu, Qianrong; Cao, Xiaoying; Ling, Chao; Xiang, Lili; Yang, Ping; Huang, Shifeng"

作者地址："[Tu, Qianrong; Ling, Chao; Yang, Ping; Huang, Shifeng] Chongqing Med Univ, Dept Clin Lab Med, Affiliated Hosp 1, 1 Friendship Rd, Chongqing 400016, Peoples R China; [Cao, Xiaoying] Chongqing Med Univ, Dept Burn & Plast Surg, Affiliated Hosp 1, 1 Friendship Rd, Chongqing 400016, Peoples R China; [Xiang, Lili] Chongqing Shapingba Dist Chenjiaqiao Hosp, Dept Clin Lab Med, Chongqing 401331, Peoples R China"

通信作者："Yang, P; Huang, SF (通讯作者)，Chongqing Med Univ, Dept Clin Lab Med, Affiliated Hosp 1, 1 Friendship Rd, Chongqing 400016, Peoples R China."

来源：AMB EXPRESS

ESI学科分类：MICROBIOLOGY

WOS号：WOS:000995963200001

JCR分区：Q2

影响因子：3.5

年份：2023

卷号：13

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：Neisseria gonorrhoeae; Recombinase polymerase amplification; CRISPR; Cas12a; Fluorescence; Lateral flow detection

摘要："Gonorrhea, caused by Neisseria gonorrhoeae (N. gonorrhoeae), is a persistent global public health threat. The development of low-cost, point-of-care testing is crucial for gonorrhea control, especially in regions with limited medical facilities. In this study, we integrated CRISPR/Cas12a reaction with recombinase polymerase amplification (RPA) to provide a simple and adaptable molecular detection method for N. gonorrhoeae. The RPA-Cas12a-based detection system developed in this study enables rapid detection of N. gonorrhoeae within 1 h without the use of specialized equipment. This method is highly specific for identifying N. gonorrhoeae without cross-reactivity with other prevalent pathogens. Furthermore, in the evaluation of 24 clinical samples, the detection system demonstrates a 100% concordance rate with traditional culture, which is being used clinically as a reference method. Overall, the RPA-Cas12a-based N. gonorrhoeae detection has the advantages of rapidity, portability, low-cost, no special equipment required, and strong operability, and has a high potential for application as a self-testing and point-of-care diagnosis, which is critical for the clinical management of gonorrhea in developing countries lacking medical equipment."

基金机构："National Natural Science Foundation of China [82072346, 82072349]; Natural Science Foundation of Chongqing, China [cstc2020jcyj-msxmX0519]"

基金资助正文："This work was sponsored by the National Natural Science Foundation of China (Grant No. 82072346 and No. 82072349) and the Natural Science Foundation of Chongqing, China (No. cstc2020jcyj-msxmX0519)."