Identification of the immune-related biomarkers in Behcet's disease by plasma proteomic analysis

作者全名："Liu, Huan; Zhang, Panpan; Li, Fuzhen; Xiao, Xiao; Zhang, Yinan; Li, Na; Du, Liping; Yang, Peizeng"

作者地址："[Liu, Huan; Li, Fuzhen; Xiao, Xiao; Zhang, Yinan; Li, Na; Du, Liping; Yang, Peizeng] Zhengzhou Univ, Henan Prov Eye Hosp, Henan Int Joint Res Lab Ocular Immunol & Retinal I, Dept Ophthalmol,Affiliated Hosp 1, Jianshe East Rd 1, Zhengzhou 450052, Henan, Peoples R China; [Liu, Huan; Xiao, Xiao; Zhang, Yinan] Zhengzhou Univ, Acad Med Sci, Zhengzhou 450052, Henan, Peoples R China; [Zhang, Panpan] Zhengzhou Univ, Dept Rheumatol & Immunol, Affiliated Hosp 1, Zhengzhou 450052, Henan, Peoples R China; [Yang, Peizeng] Chongqing Med Univ, Chongqing Key Lab Ophthalmol, Affiliated Hosp 1, Youyi Rd 1, Chongqing 400016, Peoples R China; [Yang, Peizeng] Chongqing Med Univ, Chongqing Eye Inst, Affiliated Hosp 1, Youyi Rd 1, Chongqing 400016, Peoples R China"

通信作者："Yang, PZ (通讯作者)，Zhengzhou Univ, Henan Prov Eye Hosp, Henan Int Joint Res Lab Ocular Immunol & Retinal I, Dept Ophthalmol,Affiliated Hosp 1, Jianshe East Rd 1, Zhengzhou 450052, Henan, Peoples R China.; Yang, PZ (通讯作者)，Chongqing Med Univ, Chongqing Key Lab Ophthalmol, Affiliated Hosp 1, Youyi Rd 1, Chongqing 400016, Peoples R China.; Yang, PZ (通讯作者)，Chongqing Med Univ, Chongqing Eye Inst, Affiliated Hosp 1, Youyi Rd 1, Chongqing 400016, Peoples R China."

来源：ARTHRITIS RESEARCH & THERAPY

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:000998574600001

JCR分区：Q1

影响因子：4.4

年份：2023

卷号：25

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：Behcet's disease; Uveitis; Immune; Proteomics; Biomarker

摘要："BackgroundThis study aimed to investigate the expression profile of immune response-related proteins of Behcet's disease (BD) patients and identify potential biomarkers for this disease.MethodsPlasma was collected from BD patients and healthy controls (HC). Immune response-related proteins were measured using the Olink Immune Response Panel. Differentially expressed proteins (DEPs) were used to construct prediction models via five machine learning algorithms: naive Bayes, support vector machine, extreme gradient boosting, random forest, and neural network. The prediction performance of the five models was assessed using the area under the curve (AUC) value, recall (sensitivity), specificity, precision, accuracy, F1 score, and residual distribution. Subtype analysis of BD was performed using the consensus clustering method.ResultsProteomics results showed 43 DEPs between BD patients and HC (P < 0.05). These DEPs were mainly involved in the Toll-like receptor 9 and NF-kappa B signaling pathways. Five models were constructed using DEPs [interleukin 10 (IL10), Fc receptor like 3 (FCRL3), Mannan-binding lectin serine peptidase 1 (MASP1), NF2, moesin-ezrin-radixin like (MERLIN) tumor suppressor (NF2), FAM3 metabolism regulating signaling molecule B (FAM3B), and O-6-methylguanine-DNA methyltransferase (MGMT)]. Among these models, the neural network model showed the best performance (AUC = 0.856, recall: 0.692, specificity: 0.857, precision: 0.900, accuracy: 0.750, F1 score: 0.783). BD patients were divided into two subtypes according to the consensus clustering method: one with high disease activity in association with higher expression of tripartite motif-containing 5 (TRIM5), SH2 domain-containing 1A (SH2D1A), phosphoinositide-3-kinase adaptor protein 1 (PIK3AP1), hematopoietic cell-specific Lyn substrate 1 (HCLS1), and DNA fragmentation factor subunit alpha (DFFA) and the other with low disease activity in association with higher expression of C-C motif chemokine ligand 11 (CCL11).ConclusionsOur study not only revealed a distinctive immune response-related protein profile for BD but also showed that IL10, FCRL3, MASP1, NF2, FAM3B, and MGMT could serve as potential immune biomarkers for this disease. Additionally, a novel molecular disease classification model was constructed to identify subsets of BD."

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