MicroRNA Profiling in the Aqueous Humor of Keratoconus Eyes

作者全名："Zhang, Yingjie; Che, Danyang; Cao, Yiting; Yue, Yu; He, Tianrui; Zhu, Yi; Zhou, Jibo"

作者地址："[Zhang, Yingjie; Che, Danyang; Cao, Yiting; Zhou, Jibo] Shanghai Jiao Tong Univ, Shanghai Peoples Hosp 9, Dept Ophthalmol, Sch Med, Shanghai, Peoples R China; [Zhang, Yingjie; Che, Danyang; Cao, Yiting; Zhou, Jibo] Shanghai Key Lab Orbital Dis & Ocular Oncol, Shanghai, Peoples R China; [Yue, Yu] Chongqing Med Univ, Chongqing Eye Inst, Chongqing Branch,Natl Clin Res Ctr Ocular Dis, Chongqing Key Lab Ophthalmol,Affiliated Hosp 1, Chongqing, Peoples R China; [He, Tianrui] Shanghai Jiao Tong Univ, Sch Med, Shanghai, Peoples R China; [Zhu, Yi] Aier Inst Optometry & Vis Sci, Changsha, Peoples R China; [Zhu, Yi] Shanghai Aier Eye Hosp, Shanghai, Peoples R China"

通信作者："Zhou, JB (通讯作者)，Shanghai Jiao Tong Univ, Shanghai Peoples Hosp 9, Dept Ophthalmol, Sch Med, 639 Zhizaoju Rd, Shanghai 200011, Peoples R China.; Zhu, Y (通讯作者)，Shanghai Aier Eye Hosp, 83Wuzhong Rd, Shanghai 200336, Peoples R China."

来源：TRANSLATIONAL VISION SCIENCE & TECHNOLOGY

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:001000743100014

JCR分区：Q2

影响因子：3

年份：2022

卷号：11

期号：12

开始页：　

结束页：　

文献类型：Article

关键词：microRNA; keratoconus; aqueous humor; next-generation sequencing; biomarker

摘要："Purpose: To identify differentially expressed (DE) microRNAs (miRNAs) in the aqueous humor (AH) of keratoconus (KC) eyes using next-generation sequencing and to explore whether DE miRNAs might play roles in KC pathophysiology. Methods: The small RNAs in the AH of 15 KC eyes and 15 myopia eyes (the control group) were sequenced on an Illumina NovaSeq 6000 platform. Gene Oncology and Kyoto Encyclopedia of Genes and Genome enrichment analyses were performed. Receiver operating characteristic curves were used to identify potential KC biomarkers. Results: We identified 204 miRNAs in the AH of the KC group and 200 in the AH of the control group. Fourteen miRNAs were differentially expressed between the two groups; four miRNAs were upregulated and 10 downregulated in KC AH. The possible pathways regulated by the DE miRNAs included antigen processing and presentation, endocytosis, mismatch repair, and Hippo signaling. The AH concentrations of miR-222-3p, miR-3633p, and miR-423-5p exhibited areas under the curves of 1. Conclusions: We profiled the DE miRNAs of the AH of KC eyes. These miRNAs may be associated with KC pathogenesis and could serve as KC biomarkers. Translational Relevance: Data on aberrantly expressed miRNAs in KC combined with bioinformatics analyses suggest possible roles for specific miRNAs. The DE miRNAs may serve as diagnostic KC biomarkers."

基金机构："Shanghai Municipal Health Commission [202140416]; Shanghai Ninth People's Hospital, Shanghai JiaoTong University School of Medicine [JYLJ202117]; Shanghai Municipal Commission of Health and Family Planning [2018ZHYL0221]"

基金资助正文："Supported by grants from the Shanghai Municipal Health Commission (202140416); Shanghai Ninth People's Hospital, Shanghai JiaoTong University School of Medicine (JYLJ202117); and Shanghai Municipal Commission of Health and Family Planning (2018ZHYL0221)."