A signal-on electrochemical aptasensor for sensitive detection of human epididymis protein 4 based on functionalized metal-organic framework/ketjen black nanocomposite

作者全名："Hu, Rongqin; Mu, Zhaode; Gong, Feng; Qing, Min; Yuan, Yonghua; Bai, Lijuan"

作者地址："[Hu, Rongqin; Mu, Zhaode; Yuan, Yonghua; Bai, Lijuan] Chongqing Med Univ, Chongqing Res Ctr Pharmaceut Engn, Coll Pharm, Chongqing 400016, Peoples R China; [Mu, Zhaode; Qing, Min] Chongqing Med Univ, Res Ctr Pharmacodynam Evaluat Engn Technol Chongqi, Coll Pharm, Chongqing 400016, Peoples R China; [Gong, Feng] Chongqing Ind & Informat Vocat Coll, Chongqing 400043, Peoples R China"

通信作者："Bai, LJ (通讯作者)，Chongqing Med Univ, Chongqing Res Ctr Pharmaceut Engn, Coll Pharm, Chongqing 400016, Peoples R China."

来源：JOURNAL OF MATERIALS SCIENCE

ESI学科分类：MATERIALS SCIENCE

WOS号：WOS:001000914200008

JCR分区：Q2

影响因子：3.5

年份：2023

卷号：58

期号：23

开始页：9633

结束页：9645

文献类型：Article

关键词：　

摘要："Human epididymis protein 4 (HE4) is a highly sensitive and specific biomarker for diagnosing ovarian cancer. In this work, a new electrochemical aptasensor for rapid and sensitive detection of HE4 was constructed. Firstly, metal-organic framework/ketjen black (NH2-MIL-53(Al)/KB) composite with abundant amino groups and large specific surface area was prepared as signal amplifier to anchor gold nanoparticles (AuNPs). Subsequently, signal probe (SP) and a large amount of electroactive toluidine blue (Tb) were attached on AuNPs to form the tracer label, producing a distinct electrochemical detection signal. Moreover, Ni-3(HITP)(2) was modified by poly(diallyldimethylammonium chloride, PDDA) to obtain PDDA/Ni-3(HITP)(2), and then it was covered with deposited gold nanocrystals (DpAu) to form the sensing platform, which increased the electrical conductivity, specific surface area and the loading of triple-helix assembled probes (TAP). Target HE4 was bound to its aptamer in the outer layer of the TAP, leaving a separate capture probe (CP) on the electrode surface that can bind with the tracer label. The proposed aptasensor for HE4 detection showed a wide linear range from 1 to 10 nM with a detection limit of 0.41 fM. In addition, the aptasensor has good selectivity, stability and reproducibility. More importantly, this method showed satisfactory results in clinical serum sample analysis compared with ELISA, possessing application prospects in ovarian cancer diagnosis."

基金机构："National Natural Science Foundation of China [82072378, 81601856]; Chongqing Talents: Exceptional Young Talents Project, China [CQYC202005015, cstc2021ycjh-bgzxm0328]; Ba Yu Scholar Program, China [YS2019020]; Program for Youth Innovation in Future Medicine, Chongqing Medical University, China [W0067]; Funds for High Level Young Science and Technology Talent Cultivation Plan in Chongqing Medical University, China; Discipline Talent Training Program of College of Pharmacy in Chongqing Medical University, China [YXY2019XSGG4, YXY2021BSH04]"

基金资助正文："This work is supported by National Natural Science Foundation of China (82072378, 81601856), Chongqing Talents: Exceptional Young Talents Project, China (CQYC202005015, cstc2021ycjh-bgzxm0328), Ba Yu Scholar Program, China (YS2019020), Program for Youth Innovation in Future Medicine, Chongqing Medical University, China (W0067), Funds for High Level Young Science and Technology Talent Cultivation Plan in Chongqing Medical University, China (2019) and Discipline Talent Training Program of College of Pharmacy in Chongqing Medical University, China (YXY2019XSGG4, YXY2021BSH04)."