Construction of a robust turn-on fluorescence NIR sensor for rapid detection and imaging of ONOO- in inflammatory models

作者全名："Wang, Yan; Zhao, Lulu; Xie, Liyun; Pang, Meiling; Zhang, Yazhen; Ran, Hongyan; Huang, Jianji; Wang, Junyi; Tao, Yi; Lyu, Shunqiao"

作者地址："[Wang, Yan; Lyu, Shunqiao] Chongqing Med Univ, Affiliated Hosp 1, Phase Clin Trial Ward 1, Dept Planning & Finance, Chongqing 400016, Peoples R China; [Zhao, Lulu; Xie, Liyun; Pang, Meiling; Zhang, Yazhen; Ran, Hongyan; Huang, Jianji; Wang, Junyi; Tao, Yi] Chongqing Med Univ, Coll Pharm, Int Med Coll, Chongqing 400016, Peoples R China; [Zhao, Lulu; Xie, Liyun; Pang, Meiling; Zhang, Yazhen; Ran, Hongyan; Huang, Jianji; Wang, Junyi; Tao, Yi] Chongqing Med Univ, Dept Anesthesiol, Chongqing 400016, Peoples R China"

通信作者："Lyu, S (通讯作者)，Chongqing Med Univ, Affiliated Hosp 1, Phase Clin Trial Ward 1, Dept Planning & Finance, Chongqing 400016, Peoples R China."

来源：SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY

ESI学科分类：CHEMISTRY

WOS号：WOS:001007248000001

JCR分区：Q1

影响因子：4.3

年份：2023

卷号：295

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Peroxynitrite(OONO-); Inflammatory diseases; Near-infrared; Fluorescent imaging

摘要："Peroxynitrite (OONO-) is closely related to the occurrence and development of health and inflammatory diseases. The physiological and pathological results of OONO- are related to the local concentration of ONOO-. Therefore, to develop of a simple, rapid and reliable OONO- detection tool is badly needed. In this work, we developed a small-molecule near-infrared (NIR) turn-on fluorescence sensor (NN1), harnessing a well-known response group phenylboronic acid response toward OONO-. It shows high detection sensitivity and yields a ratio (I-658/I-0) fluorescence enhancement (similar to 280-fold). In addition, NN1 can be effectively used to detect endogenous and exogenous ONOO- in living inflammatory cells. Notably, NN1 can be applied to OONO- imaging analysis in drug-induced inflammatory mice model with satisfactory results. Therefore, NN1 is a robust molecular biological tool, which has a good prospect in the study of ONOO- and the occurrence and development of inflammatory diseases."

基金机构：Chongqing Technology Inno-vation and Application Development Project [cstc2020jscx-msxmX0090]

基金资助正文：Acknowledgements This work was partially supported by Chongqing Technology Inno-vation and Application Development Project (cstc2020jscx-msxmX0090) .