DNA-scaffolded multivalent vaccine against SARS-CoV-2

作者全名："Chen, Fangfang; Huang, Yuhan; Huang, Zhengyu; Jiang, Tingting; Yang, Zailin; Zeng, Jie; Jin, Aishun; Zuo, Hua; Huang, Cheng Zhi; Mao, Chengde"

作者地址："[Chen, Fangfang; Huang, Yuhan; Huang, Zhengyu; Jiang, Tingting; Zuo, Hua; Huang, Cheng Zhi; Mao, Chengde] Southwest Univ, Key Lab Luminescence Anal & Mol Sensing, Minist Educ, Coll Pharmaceut Sci, Chongqing 400715, Peoples R China; [Yang, Zailin] Chongqing Univ, Dept Hematol Oncol, Chongqing Key Lab Translat Res Canc Metastasis & I, Canc Hosp, Chongqing, Peoples R China; [Zeng, Jie; Jin, Aishun] Chongqing Med Univ, Coll Basic Med, Dept Immunol, Chongqing, Peoples R China; [Mao, Chengde] Purdue Univ, Dept Chem, W Lafayette, IN 47907 USA"

通信作者："Huang, CZ; Mao, CD (通讯作者)，Southwest Univ, Key Lab Luminescence Anal & Mol Sensing, Minist Educ, Coll Pharmaceut Sci, Chongqing 400715, Peoples R China.; Mao, CD (通讯作者)，Purdue Univ, Dept Chem, W Lafayette, IN 47907 USA."

来源：ACTA BIOMATERIALIA

ESI学科分类：MATERIALS SCIENCE

WOS号：WOS:001008675000001

JCR分区：Q1

影响因子：9.4

年份：2023

卷号：164

期号：　

开始页：387

结束页：396

文献类型：Article

关键词：DNA nanotechnology; DNA-peptide conjugate; DNA scaffold; Multivalency; Antigenic peptide

摘要："Short peptides are poor immunogens. One way to increase their immune responses is by arraying im-munogens in multivalency. Simple and efficient scaffolds for spatial controlling the inter-antigen distance and enhancing immune activation are required. Here, we report a molecular vaccine design principle that maximally drives potent SARS-CoV-2 RBD subunit vaccine on DNA duplex to induce robust and effica-cious immune responses in vivo . We expect that the DNA-peptide epitope platform represents a facile and generalizable strategy to enhance the immune response. Statement of significance DNA scaffolds offer a biocompatible and convenient platform for arraying immunogens in multivalency antigenic peptides, and spatially control the inter-antigen distance. This can effectively enhance immune response. Peptide (instead of entire protein) vaccines are highly attractive. However, short peptides are poor immunogens. Our DNA scaffolded multivalent peptide immunogen system induced robust and ef-ficacious immune response in vivo as demonstrated by the antigenic peptide against SARS-CoV-2. The present strategy could be readily generalized and adapted to prepare multivalent vaccines against other viruses or disease. Particularly, the different antigens could be integrated into one single vaccine and lead to super-vaccines that can protect the host from multiple different viruses or multiple variants of the same virus. (c) 2023 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved."

基金机构："National Science Foundation of China [22134005, 21974111, 22274135]; Chongqing Research Program of Basic Research and Frontier Tech- nology, China [cstc2021jcyj-msxmX0931, cstc2021jcyj-msxmX1033, cstc2020jcyj-msxm X0947]; Chongqing Talents Program for Outstanding Scientists [cstc2021ycjh-bgzxm0178]; Applied Basic Research Programs of Science and Technology Department of Sichuan Province, China [2022NSFSC0682]"

基金资助正文："This work was financially supported by the National Science Foundation of China (22134005 , 21974111 and 22274135) , the Chongqing Research Program of Basic Research and Frontier Tech- nology, China (cstc2021jcyj-msxmX0931 , cstc2021jcyj-msxmX1033 , and cstc2020jcyj-msxm X0947) , Chongqing Talents Program for Outstanding Scientists (cstc2021ycjh-bgzxm0178) , and the Applied Basic Research Programs of Science and Technology Department of Sichuan Province, China (2022NSFSC0682) ."