Quality evaluation of Hawk tea from different months and regions based on quantitative analysis of multiple components with a single marker (QAMS) combined with HPLC fingerprint

作者全名："Luo, Juan; Cao, Wei-guo; Yu, Bao; Chen, Huan; Wu, Ying-qin; Li, Yang-hong; Pu, Xi-yu; Zhong, Xue; Zhang, Dang"

作者地址："[Luo, Juan; Cao, Wei-guo; Yu, Bao; Chen, Huan; Wu, Ying-qin; Li, Yang-hong; Pu, Xi-yu; Zhong, Xue; Zhang, Dang] Chongqing Med Univ, Coll Tradit Chinese Med, Chongqing 400016, Peoples R China; [Cao, Wei-guo] Chongqing Med Univ, Lab Tradit Chinese Med, Chongqing, Peoples R China"

通信作者："Zhang, D (通讯作者)，Chongqing Med Univ, Coll Tradit Chinese Med, Chongqing 400016, Peoples R China."

来源：PHYTOCHEMICAL ANALYSIS

ESI学科分类：PLANT & ANIMAL SCIENCE

WOS号：WOS:001031503500001

JCR分区：Q2

影响因子：3

年份：2023

卷号：　

期号：　

开始页：　

结束页：　

文献类型：Article; Early Access

关键词：Hawk tea; HPLC fingerprint; inclusions; QAMS; quality evaluation

摘要："BackgroundHawk tea, a medicinal and edible plant, has been consumed for thousands of years in Southwest China. To date, no unified food safety standard for Hawk tea has been established, and systematic research on the quality of Hawk tea is lacking. ObjectiveThe aim of this study was to develop a comprehensive evaluation method for the quality of Hawk tea based on inclusions content, high-performance liquid chromatography (HPLC) fingerprinting combined with the quantitative analysis of multiple components with a single marker (QAMS) method. MethodsThe contents of total flavonoids, total phenols, total polysaccharides, and total protein were determined using the colorimetric method. An effective comprehensive evaluation method was established to classify the 16 batches of samples based on HPLC fingerprint analysis combined with similarity analysis (SA), hierarchical cluster analysis (HCA), principal component analysis (PCA), partial least-squares discrimination analysis (PLS-DA), and the QAMS method. ResultsFlavonoids were the main chemical components of Hawk tea. The accuracy of the QAMS method was verified by comparing the calculated results with those of the external standard method (ESM). No significant differences were found between the two methods. Additionally, the fingerprint of Hawk tea was also established. ConclusionThe method established in this study can be used for the comprehensive quality evaluation of Hawk tea and can also provide a reference for the quality evaluation of other herbal medicines."

基金机构：Chongqing Science and Technology Bureau [cstc2020jscx-dxwtBX0054]; Chongqing Municipal Education Commission [KJZD-M202215102]; Chongqing Education Commission of China [KJQN202002801]

基金资助正文："ACKNOWLEDGEMENTS This research was founded by the Chongqing Science and Technology Bureau (cstc2020jscx-dxwtBX0054), Chongqing Municipal Education Commission (KJZD-M202215102), and Chongqing Education Commission of China (No. KJQN202002801)."