LIPUS-S/B@NPs regulates the release of SDF-1 and BMP-2 to promote stem cell recruitment-osteogenesis for periodontal bone regeneration

作者全名："Yan, Shujin; Wang, Dong; Zhang, Liang; Gan, Tian; Yao, Huan; Zhu, Hui; He, Yiman; Yang, Ke"

作者地址："[Yan, Shujin; Yang, Ke] Chongqing Med Univ, Childrens Hosp, Chongqing Engn Res Ctr Stem Cell Therapy,Minist Ed, China Int Sci & Technol Cooperat Base Child Dev &, Chongqing, Peoples R China; [Yan, Shujin; Wang, Dong; Zhang, Liang; Yao, Huan; Zhu, Hui; He, Yiman] Chongqing Med Univ, Affiliated Hosp 1, Dept Ultrasound, Chongqing, Peoples R China; [Gan, Tian] Chongqing Med Univ, Women & Childrens Hosp, Dept Ultrasound, Chongqing, Peoples R China"

通信作者："Yang, K (通讯作者)，Chongqing Med Univ, Childrens Hosp, Chongqing Engn Res Ctr Stem Cell Therapy,Minist Ed, China Int Sci & Technol Cooperat Base Child Dev &, Chongqing, Peoples R China."

来源：FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY

ESI学科分类：Multidisciplinary

WOS号：WOS:001031570400001

JCR分区：Q1

影响因子：4.3

年份：2023

卷号：11

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：LIPUS; nanoparticle; stem cell; recruitment-osteogenesis; periodontal bone regeneration

摘要："Purpose: Poly (lactic-co-glycolic acid)-based nanoparticles (PLGA NPs) have been widely used as the carrier for sustainable drug delivery. However, the drug release from the NPs was usually incomplete and uncontrollable. Herein, a low intensity pulsed ultrasound (LIPUS) assisted SDF-1/BMP-2@nanoparticles (S/B@NPs) system was fabricated to facilitate stem cell recruitment-osteogenesis for periodontal bone regeneration.Methods: In this work, S/B@NPs were prepared with double-emulsion synthesis method. Then the S/B release profile from NPs was evaluated with or without low intensity pulsed ultrasound treatment. Afterwards, the stem cell recruiting and osteoinductive capacities of LIPUS-S/B@NPs were detected with human periodontal ligament cells (hPDLCs) in vitro and in a rat periodontal bone defect model.Results: The results indicated that S/B@NPs were successfully prepared and LIPUS could effectively regulate the release of S/B and increase their final releasing amount. Moreover, LIPUS-S/B@NPs system significantly promoted hPDLCs migrating and osteogenesis in vitro and recruiting rBMSCs to the rat periodontal defect and facilitated bone regeneration in vivo.Conclusion: Our LIPUS assisted S/B@NPs system can effectively facilitate stem cell recruitment and periodontal bone regeneration. Considering its reliable safety and therapeutic effect on bone fracture, LIPUS, as an adjuvant therapy, holds great potential in the regulation of drug delivery systems for bone healing."

基金机构：Natural Science Foundation Project of CQ [CSTB2022NSCQ-MSX0812]

基金资助正文：This work was supported by the Natural Science Foundation Project of CQ (Grant No. CSTB2022NSCQ-MSX0812).