High C1QTNF1 expression mediated by potential ncRNAs is associated with poor prognosis and tumor immunity in kidney renal clear cell carcinoma

作者全名："Qiu, Jiechuan; Wang, Zicheng; Zhao, Leizuo; Zhang, Peizhi; Xu, Yingkun; Xia, Qinghua"

作者地址："[Qiu, Jiechuan; Wang, Zicheng; Xia, Qinghua] Shandong First Med Univ, Shandong Prov Hosp, Dept Urol, Jinan, Peoples R China; [Zhao, Leizuo] Dongying Peoples Hosp, Dept Urol, Dongying, Peoples R China; [Zhao, Leizuo; Zhang, Peizhi] Shandong Univ, Shandong Prov Hosp, Cheeloo Coll Med, Dept Urol, Jinan, Peoples R China; [Xu, Yingkun] Chongqing Med Univ, Affiliated Hosp 1, Dept Breast & Thyroid Surg, Chongqing, Peoples R China"

通信作者："Xia, QH (通讯作者)，Shandong First Med Univ, Shandong Prov Hosp, Dept Urol, Jinan, Peoples R China."

来源：FRONTIERS IN MOLECULAR BIOSCIENCES

ESI学科分类：BIOLOGY & BIOCHEMISTRY

WOS号：WOS:001040057600001

JCR分区：Q2

影响因子：3.9

年份：2023

卷号：10

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：clear cell renal cell carcinoma; C1QTNF1; ceRNA; prognostic markers; adipokines

摘要："Background: Kidney renal clear cell carcinoma (KIRC) originates from proximal tubular cells and is the most common subtype of renal cell carcinoma. KIRC is characterized by changes in lipid metabolism, and obesity is a risk factor for it. C1q And TNF Related 1 (C1QTNF1), a novel adipokine and member of the C1q and TNF-related protein (CTRP) family, has been shown to affect the progression of various cancers. However, the role of C1QTNF1 in KIRC has not been studied.Methods: The Wilcoxon rank sum test was used to analyze the expression of C1QTNF1 in KIRC tissues and normal tissues. The relationship between clinicopathological features and C1QTNF1 levels was also examined by logistic regression and the Wilcoxon rank sum test. In addition, the effect of C1QTNF1 on the prognosis of KIRC patients was analyzed by Kaplan-Meier (KM). The Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) were used to analyze the potential signaling pathways and biological functions of differential genes. A nomogram was constructed to predict the prognosis of KIRC patients. Spearman correlation analysis was performed to determine the association between C1QTNF1 expression and immune cell infiltration and immune checkpoint genes. The upstream miRNAs and lncRNAs of C1QTNF1 were predicted by the ENCORI online tool. Finally, we examined the proliferation, invasion, and migration abilities of KIRC cells after C1QTNF1 knockdown.Results: The expression of C1QTNF1 in KIRC tissues was significantly higher than in normal renal tissues. Patients with higher C1QTNF1 expression had a poor prognosis, a finding supported by Kaplan-Meier survival analysis. C1QTNF1 expression was significantly correlated with TNM and pathologic stages, age, and gender (p < 0.05). The C1QTNF1 expression level was significantly correlated with immune cell infiltration and immune checkpoint genes in KIRC. Additionally, high C1QTNF1 expression was associated with poor prognosis in stage I and II, T1 and T2, T3 and T4, N0, and M0 patients (HR > 1, p < 0.05). The calibration diagram shows that the C1QTNF1 model has effective predictive performance for the survival of KIRC patients. Knockdown of C1QTNF1 inhibited KIRC cell proliferation, cell migration, and cell invasion. In addition, CYTOR and AC040970.1/hsa-miR-27b-3p axis were identified as the most likely upstream ncRNA-related pathways of C1QTNF1 in KIRC.Conclusion: In conclusion, our study suggests that high expression of C1QTNF1 is associated with KIRC progression and immune infiltration. The increased expression of C1QTNF1 suggests a poor prognosis in KIRC patients."

基金机构："National Natural Science Foundation of China [82072816, 82272713]; Natural Science Foundation of Shandong Province [ZR2021LZY003]"

基金资助正文：& nbsp;This research was funded by the National Natural Science Foundation of China (Grant Nos. 82072816 and 82272713) and the Natural Science Foundation of Shandong Province (Grant No. ZR2021LZY003).