Change Profiles and Functional Targets of MicroRNAs in Type 2 Diabetes Mellitus Patients with Obesity

作者全名："Lu, Guanhua; Gao, Huanhuan; Dong, Zhiyong; Jiang, Shuwen; Hu, Ruixiang; Wang, Cunchuan"

作者地址："[Lu, Guanhua; Dong, Zhiyong; Jiang, Shuwen; Hu, Ruixiang; Wang, Cunchuan] Jinan Univ, Affiliated Hosp 1, Dept Metab & Bariatr Surg, Guangzhou, Peoples R China; [Lu, Guanhua; Dong, Zhiyong; Jiang, Shuwen; Hu, Ruixiang; Wang, Cunchuan] Guangdong Hong Kong Macao Joint Univ Lab Metab & M, Guangzhou, Peoples R China; [Gao, Huanhuan] Chongqing Med Univ, Affiliated Hosp 3, Dept Ophthalmol, Chongqing, Peoples R China; [Dong, Zhiyong; Wang, Cunchuan] Jinan Univ, Inst Obes & Metab Disorders, Guangzhou, Peoples R China; [Hu, Ruixiang; Wang, Cunchuan] Jinan Univ, Affiliated Hosp 1, Dept Metab & Bariatr Surg, 613 Huangpu Ave West, Guangzhou, Guangdong, Peoples R China"

通信作者："Hu, RX; Wang, CC (通讯作者)，Jinan Univ, Affiliated Hosp 1, Dept Metab & Bariatr Surg, 613 Huangpu Ave West, Guangzhou, Guangdong, Peoples R China."

来源：DIABETES & METABOLISM JOURNAL

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:001043141500010

JCR分区：Q1

影响因子：6.8

年份：2023

卷号：47

期号：4

开始页：559

结束页：570

文献类型：Article

关键词：T2DM; Bariatric surgery; Diabetes mellitus; type 2; Metabolism; MicroRNAs; Obesity

摘要："Background: MicroRNAs (miRNAs) exert an essential contribution to obesity and type 2 diabetes mellitus (T2DM). This study aimed to investigate the differences of miRNAs in the presence and absence of T2DM in patients with obesity, as well as before and after bariatric surgery in T2DM patients with obesity. Characterization of the common changes in both was further analyzed.Methods: We enrolled 15 patients with obesity but without T2DM and 15 patients with both obesity and T2DM. Their preopera-tive clinical data and serum samples were collected, as well as 1 month after bariatric surgery. The serum samples were analyzed by miRNA sequencing, and the miRNAs profiles and target genes characteristics were compared.Results: Patients with T2DM had 16 up-regulated and 32 down-regulated miRNAs compared to patients without T2DM. Im-provement in metabolic metrics after bariatric surgery of T2DM patients with obesity was correlated with changes in miRNAs, as evidenced by the upregulation of 20 miRNAs and the downregulation of 30 miRNAs. Analysis of the two miRNAs profiles identi-fied seven intersecting miRNAs that showed opposite changes. The target genes of these seven miRNAs were substantially en-riched in terms or pathways associated with T2DM. Conclusion: We determined the expression profiles of miRNAs in the obese population, with and without diabetes, before and after bariatric surgery. The miRNAs that intersected in the two comparisons were discovered. Both the miRNAs discovered and their target genes were closely associated with T2DM, demonstrating that they might be potential targets for the regulation of T2DM."

基金机构：flagship specialty construction project-general surgery of The First Affiliated Hospital of Jinan University [711003]; First Affiliated Hospital of Jinan University self-raised talent introduction research start-up fee [702016]

基金资助正文：This research was sponsored by the flagship specialty construction project-general surgery of The First Affiliated Hospital of Jinan University (Funding No.: 711003) and The First Affiliated Hospital of Jinan University self-raised talent introduction research start-up fee (Funding No.: 702016).