Gold-plated PCN-222(Fe) and superconductive carbon black-based sandwich-type immunosensor for detecting CYFRA21-1

作者全名："Fu, Lin; Mu, Zhaode; Zhou, Jing; Qing, Min; Bai, Lijuan"

作者地址："[Fu, Lin; Zhou, Jing; Bai, Lijuan] Chongqing Med Univ, Coll Pharm, Chongqing Res Ctr Pharmaceut Engn, Chongqing 400016, Peoples R China; [Mu, Zhaode; Qing, Min] Chongqing Med Univ, Coll Pharm, Res Ctr Pharmacodynam Evaluat Engn Technol Chongqi, Chongqing 400016, Peoples R China"

通信作者："Bai, LJ (通讯作者)，Chongqing Med Univ, Coll Pharm, Chongqing Res Ctr Pharmaceut Engn, Chongqing 400016, Peoples R China.; Qing, M (通讯作者)，Chongqing Med Univ, Coll Pharm, Res Ctr Pharmacodynam Evaluat Engn Technol Chongqi, Chongqing 400016, Peoples R China."

来源：JOURNAL OF MATERIALS CHEMISTRY B

ESI学科分类：MATERIALS SCIENCE

WOS号：WOS:001047738900001

JCR分区：Q1

影响因子：6.1

年份：2023

卷号：　

期号：　

开始页：　

结束页：　

文献类型：Article; Early Access

关键词：　

摘要："Cytokeratin 19 fragment antigen 21-1 (CYFRA21-1) is a protein fragment dissolved in the blood after apoptosis of lung epithelial cells, which is a predictive biomarker for the diagnosis of non-small cell lung cancer (NSCLC). Detection of serum CYFRA21-1 has a significant clinical value in diagnosis, monitoring and prognosis of NSCLC. Herein, a novel electrochemical immunosensor was constructed for the sensitive detection of CYFRA21-1. First, superconductive carbon black (KB) functionalized polyethyleneimine (PEI)-gold nanoparticles (AuNPs) were covered on the surface of methylene blue (MB) and used as substrate materials to immobilize the CYFRA21-1 antibody. Then, target CYFRA21-1 was successfully detected using an electrochemical immunosensor through specific recognition of antigen and antibody. The zirconium-based metal organic framework of PCN-222(Fe) with a large pore size and three-dimensional (3D) structure can absorb abundant AuNPs through strong electrostatic interaction, which enhances the conductive properties of PCN-222(Fe) and prevents the self-aggregation of AuNPs. However, PCN-222(Fe) with peroxidase-like activity can catalyze the generation of hydroxyl free radicals (OH) from H2O2, which oxidized MB, leading to a decrease in the current signal. The signal response to the degradation of MB was recorded using differential pulse voltammetry (DPV). This indirect method of immunosensor offered a new strategy to address the limitations imposed by the poor conductivity of PCN-222(Fe), further enabling the amplification of the signal through the oxidative degradation of MB. Compared with traditional electrochemical immunosensors, this method has the advantages of a stable current signal and good reproducibility, providing a promising reference for the broad application of PCN-222(Fe) in electrochemical biosensors."

基金机构："National Natural Science Foundation of China [82072378, 81601856]; Chongqing Talents: Exceptional Young Talents Project, China [CQYC202005015, cstc2021ycjh-bgzxm0328]; Ba Yu Scholar Program of Chongqing, China [YS2019020]; Program for Youth Innovation in Future Medicine, Chongqing Medical University, China [W0067]; Funds for High Level Young Science and Technology Talent Cultivation Plan in Chongqing Medical University, China; Discipline Talent Training Program of College of Pharmacy in Chongqing Medical University, China [YXY2019XSGG4, YXY2021BSH04]; Natural Science Foundation of Chongqing, China [CSTB2022NSCQ-BHX0006]; Special Funding for Postdoctoral Research Project of Chongqing, China [2021XM2045]; 2022 Graduate Research and Innovation Project in Chongqing, China [CYS22369]"

基金资助正文："This work is supported by National Natural Science Foundation of China (82072378, 81601856), Chongqing Talents: Exceptional Young Talents Project, China(CQYC202005015, cstc2021ycjh-bgzxm0328), Ba Yu Scholar Program of Chongqing, China (YS2019020), Program for Youth Innovation in Future Medicine, Chongqing Medical University, China (W0067), Funds for High Level Young Science and Technology Talent Cultivation Plan in Chongqing Medical University, China(2019) and Discipline Talent Training Program of College of Pharmacy in Chongqing Medical University, China (YXY2019XSGG4, YXY2021BSH04). Natural Science Foundation of Chongqing, China (CSTB2022NSCQ-BHX0006), Special Funding for Postdoctoral Research Project of Chongqing, China (2021XM2045), 2022 Graduate Research and Innovation Project in Chongqing, China (CYS22369)."