Smartphone and paper-based device for glucose monitoring using acetylene black-hemin nanozyme as catalyst

作者全名："Yi, Xiaojuan; Yuan, Yonghua; Qing, Min; Wang, Lulu; Li, Huizhen; Bai, Lijuan"

作者地址："[Yi, Xiaojuan; Wang, Lulu; Li, Huizhen; Bai, Lijuan] Chongqing Med Univ, Coll Pharm, Chongqing Res Ctr Pharmaceut Engn, Chongqing 400016, Peoples R China; [Yuan, Yonghua; Qing, Min] Chongqing Med Univ, Coll Pharm, Res Ctr Pharmacodynam Evaluat Engn Technol Chongqi, Chongqing 400016, Peoples R China"

通信作者："Bai, LJ (通讯作者)，Chongqing Med Univ, Coll Pharm, Chongqing Res Ctr Pharmaceut Engn, Chongqing 400016, Peoples R China."

来源：SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY

ESI学科分类：CHEMISTRY

WOS号：WOS:001058504300001

JCR分区：Q1

影响因子：4.3

年份：2023

卷号：296

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Blood glucose; Nanozyme; Paper-based analytical device; Acetylene black; Hemin

摘要："Glucose management is an important part of disease control for diabetes patients, thus the development of a rapid and real-time point of care testing (POCT) device for monitoring blood glucose is of great significance. In this work, a paper-based analytical device (PAD) is constructed by combining acetylene black (AB)-hemin complex modified filter paper as sensing platform with a smartphone as signal detector. Large specific surface area of AB decreases the self-associate and aggregate of hemin in aqueous solution, resulting in improved peroxidase-like activity of hemin. Compared with graphene oxide supported hemin, AB-hemin exhibits superior signal response on paper. Glucose oxidase (GOx) catalyzes the conversion of blood glucose to hydrogen peroxide, and then AB-hemin complex catalyzes the oxidation of colorless 3,3 ',5,5 '-tetramethylbenzidine (TMB) to blue TMB oxidized products (TMB+) in the presence of hydrogen peroxide, thus achieving the visual detection of blood glucose. In optimal conditions, PAD provides an applicable linear range from 0.2 mM to 30 mM and a low limit of detection (LOD) (0.06 mM). Notably, the detection accuracy of the developed paper-based sensor is in good agreement with that of the commercially available blood glucose meter (p > 0.05). Moreover, the proposed PAD presents high recoveries from 95.4% to 112% (RSD <= 3.2%), and therefore holds great potential for glucose monitoring and diabetes diagnosis."

基金机构："National Natural Science Foundation of China, China [81601856, CQYC202005015]; Chongqing Talents: Exceptional Young Talents Project, China [cstc2021ycjh-bgzxm0328, W0067]; Program for Youth Innovation in Future Medicine, Chongqing Medical Univer-sity, China [YXY2019XSGG4]; Funds for High Level Young Science and Tech-nology Talent Cultivation Plan in Chongqing Medical University, China; Natural Science Foundation of Chongqing, China [YXY2021BSH04]; Discipline Talent Training Program of College of Pharmacy in Chongqing Medical University, China [cstc2014kjrc-qnrc00004, YS2019020]; Funds for Young Science and Technology Talent Cultivation Plan of Chongqing City, China; Ba Yu Scholar Program, China; [82072378]; [CSTB2022NSCQ-BHX0006]"

基金资助正文："This work is supported by National Natural Science Foundation of China, China (82072378, 81601856) , Chongqing Talents: Exceptional Young Talents Project, China (CQYC202005015, cstc2021ycjh-bgzxm0328) , Ba Yu Scholar Program, China (YS2019020) , Program for Youth Innovation in Future Medicine, Chongqing Medical University, China (W0067) , Funds for High Level Young Science and Technology Talent Cultivation Plan in Chongqing Medical University, China (2019) , Natural Science Foundation of Chongqing, China (CSTB2022NSCQ-BHX0006) , Discipline Talent Training Program of College of Pharmacy in Chongqing Medical University, China (YXY2019XSGG4, YXY2021BSH04) and Funds for Young Science and Technology Talent Cultivation Plan of Chongqing City, China (cstc2014kjrc-qnrc00004) ."