Quality Evaluation of Bioactive Ingredients in Lianhua Qingwen Capsule Based on Quantitative Analysis of Multicomponent by the Single Marker Method and the Chemical Recognition Patterns Method

作者全名："Zhou, Qianqian; Yang, Lin; Wang, Yiwu; Zou, Jiajia; Li, Shuya; Dai, Lei; Li, Yan; He, Dan"

作者地址："[Zhou, Qianqian; Zou, Jiajia; Li, Shuya; Dai, Lei; He, Dan] Chongqing Med Univ, Coll Pharm, Chongqing 400016, Peoples R China; [Yang, Lin; Li, Yan] Chongqing Med & Pharmaceut Coll, Chongqing Pharmaceut Preparat Engn Technol Res Ctr, Chongqing 401331, Peoples R China; [Wang, Yiwu] Chongqing Med Univ, Expt Teaching Ctr, Chongqing 400016, Peoples R China"

通信作者："He, D (通讯作者)，Chongqing Med Univ, Coll Pharm, Chongqing 400016, Peoples R China."

来源：JOURNAL OF CHEMISTRY

ESI学科分类：CHEMISTRY

WOS号：WOS:001064567500001

JCR分区：Q2

影响因子：2.8

年份：2023

卷号：2023

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：　

摘要："Based on high performance liquid chromatography with the diode array detector (HPLC-DAD), a new strategy for simultaneous determination of ten bioactive ingredients in Lianhua Qingwen capsule (LHQW) was developed for comprehensive quality assessment of LHQW. In this work, with rhein regarded as the internal reference substance (IRS), the relative correction factors (RCFs) of neochlorogenic acid, amygdalin, chlorogenic acid, forsythoside A, quercitrin, phillyin, glycyrrhizic acid, iso-forsythiaside, and (+) pinoresinol-beta-D-glucoside were calculated for simultaneous determination of ten bioactive ingredients. More importantly, compared to previous work, the simultaneous determination of the content of ten pharmacologically important active ingredients at one detection wavelength with only one reference substance has been achieved. Based on the contents of ten bioactive ingredients, the quality of the 20 batches of LHQW samples was further analyzed by chemical recognition patterns method. Ten bioactive ingredients showed a good linear relationship in their respective concentration ranges (r >= 0.999).)e relative standard deviations (RSDs) of precision (<= 4.62%), stability (<= 4.04%), repeatability (<= 3.87%), and the average recovery of ten bioactive components (99.8%similar to 104.1%) demonstrated the QAMS developed for LHQW which had good durability.)e correlation coeAcient ( P > 0.05) showed that no signiCcant diDerence existed in the results of QAMS and external standard method (ESM). Hierarchical clustering analysis (HCA) divided samples into three main groups. Radar plot analysis and principal component analysis (PCA) found some quality diDerences existed between the three groups of samples. Orthogonal partial least-squares discrimination analysis (OPLS-DA) showed that forsythoside A could be used as the primary marker responsible for the quality diDerences. In conclusion, the established QAMS method combined with chemometric analysis can simultaneously determine the content of 10 active components and comprehensively evaluate the quality of diDerent batches of LHQW. It can provide scientiCc basis and reference of quality consistency evaluation for the formulation manufacturers and drug regulatory authorities."

基金机构：Science and Technology Research Project of Chongqing Education Commission [KJQN202202807]; General Project of Chongqing Science and Technological Committee [CSTB2022NSCQ-MSX0966]; Chongqing Medical University Personnel Support Program [YXY2021SDTR01]

基金资助正文："This research was supported by grants from the Science and Technology Research Project of Chongqing Education Commission (Grant no. KJQN202202807), General Project of Chongqing Science and Technological Committee (Grant no. CSTB2022NSCQ-MSX0966), and Chongqing Medical University Personnel Support Program (Grant no. YXY2021SDTR01)."