Experimental study of HIFU incomplete ablation on the damage effect and prognosis of rabbit VX2 breast cancer model

作者全名："Feng, Xiaoling; Yang, Min; Li, Jianhu; Liao, Hongjian; Zhang, Zhifei; Wang, Qi; Du, Yonghong"

作者地址："[Feng, Xiaoling; Yang, Min; Li, Jianhu; Liao, Hongjian; Zhang, Zhifei; Wang, Qi; Du, Yonghong] Chongqing Med Univ, Coll Biomed Engn, State Key Lab Ultrasound Med & Engn, Chongqing, Peoples R China; [Feng, Xiaoling; Yang, Min; Li, Jianhu; Liao, Hongjian; Zhang, Zhifei; Wang, Qi; Du, Yonghong] Chongqing Med Univ, Chongqing Key Lab Biomed Engn, Chongqing, Peoples R China; [Wang, Qi; Du, Yonghong] Chongqing Med Univ, Coll Biomed Engn, State Key Lab Ultrasound Med & Engn, Chongqing 400016, Peoples R China"

通信作者："Wang, Q; Du, YH (通讯作者)，Chongqing Med Univ, Coll Biomed Engn, State Key Lab Ultrasound Med & Engn, Chongqing 400016, Peoples R China."

来源：INTERNATIONAL JOURNAL OF HYPERTHERMIA

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:001067213600001

JCR分区：Q2

影响因子：3

年份：2023

卷号：40

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：HIFU incomplete ablation; breast cancer; apoptosis; anti-tumor immune; metastasis

摘要："Purpose High-intensity focused ultrasound (HIFU) represents an emerging noninvasive modality for tumor treatment. While biological responses and immunological change associated with incomplete ablation have not been thoroughly investigated. This study aims to evaluate the damage effect of HIFU incomplete ablation via establishing animal model and further explore its possible mechanism to inhibit tumor growth.Methods The rabbit VX2 breast cancer model was established and received HIFU treatment with complete ablation (100% tumor volume) and incomplete ablation (about 80% tumor volume) under real-time B-ultrasound monitoring. Histopathological alterations, dynamics of tumor cell apoptosis and proliferation, expression levels of VEGF, MMP-9, IL-2R, TGF-& beta;1, HSP-70, IL-6, IL-8, and INF-& gamma;, and the presence of circulating tumor cells (CTCs) were evaluated post-HIFU incomplete ablation.Results For HIFU 80% ablation group, there was an 85.85% reduction in tumor volume 21 days post-intervention. A marked increase in tumor cell apoptosis and a concomitant decrease in proliferation were observed. Notably, distant tumor metastasis rates, CTC counts, and expression levels of VEGF, MMP-9, IL-2R, TGF-& beta;1, IL-6, and IL-8 were significantly reduced. In contrast, INF-& gamma; and HSP-70 expressions were notably elevated, aligning with findings from the 100% ablation group.Conclusions HIFU incomplete ablation, with an 80% tumor ablation rate, induces substantial tumor damage, augments tumor cell apoptosis, and triggers an anti-tumor immune response, curtailing metastasis. These insights may underpin further investigations into the therapeutic implications of HIFU incomplete ablation."

基金机构：Chongqing Graduate Research Innovation Project Fund [CYS20213]

基金资助正文："Thanks to the State Key Laboratory of Ultrasound in Medicine and Engineering, College of Biomedical Engineering for providing the experimental platform. Thanks to the members of this research group, advisors and mentors for their help. Thanks to the support of the Chongqing Graduate Research Innovation Project Fund (No. CYS20213)."