Proteomic profiling analysis of human endometrium in women with unexplained recurrent spontaneous abortion
作者全名："Zhang, Lei; Li, Qian; Su, Yan; Zhang, Xinyuan; Qu, Jialin; Liao, Dan; Zou, Qin; Zou, Hua; Liu, Xiaoli; Li, Chunli; He, Junlin"
作者地址："[Zhang, Lei; Li, Qian; Su, Yan; Zhang, Xinyuan; Qu, Jialin; Liao, Dan; Zou, Qin; Zou, Hua; Li, Chunli] Chongqing Hlth Ctr Women & Children, Dept Clin Lab, Chongqing, Peoples R China; [Zhang, Lei; Li, Qian; Su, Yan; Zhang, Xinyuan; Qu, Jialin; Liao, Dan; Zou, Qin; Zou, Hua; Li, Chunli] Chongqing Med Univ, Women & Childrens Hosp, Dept Clin Lab, Chongqing, Peoples R China; [Liu, Xiaoli] Chongqing Hlth Ctr Women & Children, Dept Family Planning, Chongqing, Peoples R China; [He, Junlin] Chongqing Med Univ, Sch Publ Hlth, Chongqing 400016, Peoples R China; [He, Junlin] Chongqing Med Univ, Joint Int Res Lab Reprod & Dev, Chongqing 400016, Peoples R China; [He, Junlin] Chongqing Med Univ, 1 Yixueyuan Rd, Chongqing 400016, Peoples R China; [Liu, Xiaoli; Li, Chunli] Chongqing Hlth Ctr Women & Children, 120 Longshan Rd, Chongqing 401147, Peoples R China"
通信作者："He, JL (通讯作者)，Chongqing Med Univ, 1 Yixueyuan Rd, Chongqing 400016, Peoples R China.; Liu, XL; Li, CL (通讯作者)，Chongqing Hlth Ctr Women & Children, 120 Longshan Rd, Chongqing 401147, Peoples R China."
来源：JOURNAL OF PROTEOMICS
ESI学科分类：BIOLOGY & BIOCHEMISTRY
WOS号：WOS:001074218100001
JCR分区：Q2
影响因子：2.8
年份：2023
卷号：288
期号：
开始页：
结束页：
文献类型：Article
关键词：Recurrent spontaneous abortion; Proteomics; Endometrium; Decidualization
摘要："Unexplained recurrent spontaneous abortion (URSA) seriously affects female reproductive health, causing a great burden to patients both physically and mentally. Endometrial decidualization plays an important role in pregnancy, and impaired decidualization is an essential cause of URSA, but the cause of the damage is still poorly understood. This study aimed to reveal the pathogenesis of URSA by analyzing the differential protein expression profiles in the decidual tissue of patients with recurrent abortion compared to those with normal pregnancy. Morphological analysis revealed abnormal decidualization of endometrial tissue in patients with URSA. Quantitative proteomics analysis showed that a total of 146 differentially expressed proteins were identified between the two groups, among which 95 proteins were downregulated and 51 proteins were upregulated. Gene Ontology (GO) terms and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways revealed that the protein expression profile and signaling pathways of endometrium in patients with URSA changed significantly, and cytoskeleton remodeling and morphological transformation disorders were associated with abortion induced by incomplete decidualization. Meanwhile, transcription factors analysis showed that the 3 most affected families were zf-C2H2, MYB and HMG. Therefore, our study may provide a basis for searching for potential markers of decidualization injury.Significance: At present, there are still about 50% of RSA patients with unknown causes, which brings great difficulties and blindness to clinical diagnosis and treatment.The limited proteomic studies on URSA further contribute to the lack of understanding in this field. However, in this study, the focus was on proteomic profiling analysis of the human endometrium in URSA patients compared to normal women. The findings revealed that cytoskeletal remodeling disorder is a significant contributor to the failure of decidualization in URSA patients. This insight highlights the potential role of cytoskeleton-related proteins in the pathogenesis of URSA, providing valuable information for further research and potential therapeutic interventions."
基金机构："Chongqing Postdoctoral Science Foundation [CSTB2022NSCQ-BHX0012, CSTB2022NSCQ-BHX0692]; Natural Science Foundation Project of Chongqing, Chongqing Science and Technology Commission [cstc2020jcyj-msxmX0443]; Youth Cultivation Project of Chongqing Health Center for Women and Children [2021YJQN05]"
基金资助正文："This study was supported by Chongqing Postdoctoral Science Foundation (CSTB2022NSCQ-BHX0012, CSTB2022NSCQ-BHX0692) , Natural Science Foundation Project of Chongqing, Chongqing Science and Technology Commission (cstc2020jcyj-msxmX0443) , Youth Cultivation Project of Chongqing Health Center for Women and Children (2021YJQN05) ."
