Liposomal Resveratrol Alleviates Platelet Storage Lesion via Antioxidation and the Physical Buffering Effect

作者全名："Hou, Jiazhen; Xiong, Wei; Shao, Xinyue; Lang, Li; Chang, Ya; Chen, Guihua; Wang, Li; Wang, Zhicheng; Huang, Yongzhuo"

作者地址："[Hou, Jiazhen; Huang, Yongzhuo] Nanjing Univ Chinese Med, Sch Chinese Mat Med, Nanjing 210023, Peoples R China; [Hou, Jiazhen; Shao, Xinyue; Lang, Li; Chang, Ya; Chen, Guihua; Huang, Yongzhuo] Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China; [Xiong, Wei; Huang, Yongzhuo] Chinese Acad Sci, Shanghai Inst Mat Med, Zhongshan Inst Drug Discovery, Zhongshan 528437, Peoples R China; [Xiong, Wei; Lang, Li; Chen, Guihua] Guangzhou Univ Chinese Med, Artemisinin Res Ctr, Guangzhou 510450, Peoples R China; [Wang, Li; Wang, Zhicheng] Fudan Univ, Shanghai Med Coll, Huashan Hosp, Dept Transfus Med, Shanghai 200040, Peoples R China; [Wang, Li] Chongqing Med Univ, Ctr Clin Mol Med Detect, Affiliated Hosp 1, Chongqing 400016, Peoples R China; [Huang, Yongzhuo] NMPA Key Lab Qual Res & Evaluat Pharmaceut Excipi, Shanghai 201203, Peoples R China"

通信作者："Huang, YZ (通讯作者)，Nanjing Univ Chinese Med, Sch Chinese Mat Med, Nanjing 210023, Peoples R China.; Huang, YZ (通讯作者)，Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China.; Huang, YZ (通讯作者)，Chinese Acad Sci, Shanghai Inst Mat Med, Zhongshan Inst Drug Discovery, Zhongshan 528437, Peoples R China.; Wang, ZC (通讯作者)，Fudan Univ, Shanghai Med Coll, Huashan Hosp, Dept Transfus Med, Shanghai 200040, Peoples R China.; Huang, YZ (通讯作者)，NMPA Key Lab Qual Res & Evaluat Pharmaceut Excipi, Shanghai 201203, Peoples R China."

来源：ACS APPLIED MATERIALS & INTERFACES

ESI学科分类：MATERIALS SCIENCE

WOS号：WOS:001075421900001

JCR分区：Q1

影响因子：8.3

年份：2023

卷号：15

期号：39

开始页：45658

结束页：45667

文献类型：Article

关键词：platelet storage lesion; platelet storage; liposome; resveratrol; antioxidant

摘要："Platelet transfusion is essential in the treatment of platelet-related diseases and the prevention of bleeding in patients with surgical procedures. Platelet transfusion efficacy and shelf life are limited mainly by the development of platelet storage lesion (PSL). Mitigating PSL is the key to prolonging the platelet shelf life and reducing wastage. Excess intracellular reactive oxygen species (ROS) are one of the main factors causing PSL. In this study, we explored a nanomedicine strategy to improve the quality and functions of platelets in storage. Resveratrol (Res), a natural plant product, is known for its antioxidative effect. However, medical applications of Res are limited due to its low water solubility and stability. Therefore, we used a resveratrol-loaded liposomal system (Res-Lipo) to better utilize the antioxidant effect of the drug. This study aimed to evaluate the effect of Res-Lipo on platelet oxidative stress and alleviation of PSL during the storage time. Res-Lipo scavenged intracellular ROS and inhibited platelet apoptosis and activation during storage. Res-Lipo not only maintained mitochondrial function but also improved platelet aggregation in response to adenosine 5 ' -diphosphate. These results revealed that Res-Lipo ameliorated PSL and prolonged the platelet survival time in vivo. The strategy provides a potential method for extending the platelet storage time and might be considered a potential and safe additive to alleviate PSL."

基金机构："National Key Research and Development Program of China (China) [2021YFC2400600, 2021YFE0103100]; NFSC (China) [81925035]; National Foreign Expert Program [82050410361]; Chinese Pharmaceutical Association-Yiling Joint Project [CPAYLJ201901]; High Level New R&D Institute from the Department of Science and Technology of Guangdong Province [2019B090904008]; High-Level Innovative Research Institute from the Department of Science and Technology of Guangdong Province [2021B0909050003]; Zhongshan Municipal Bureau of Science and Technology [LJ2021001, CXTD2022011]"

基金资助正文："This work was supported by the National Key Research and Development Program of China (2021YFC2400600, 2021YFE0103100, China), NFSC (81925035, China), National Foreign Expert Program (82050410361), Chinese Pharmaceutical Association-Yiling Joint Project (CPAYLJ201901), HighLevel New R&D Institute (2019B090904008) and High-Level Innovative Research Institute (2021B0909050003) from the Department of Science and Technology of Guangdong Province, and Zhongshan Municipal Bureau of Science and Technology (LJ2021001 & CXTD2022011). We also thank the Molecular Imaging Center and TEM Facility at SIMM and National Center for Protein Science Shanghai, CAS."