GC-MS based comparative metabolomic analysis of human cancellous bone reveals the critical role of linoleic acid metabolism in femur head necrosis

作者全名："Zhu, Weiwen; Wang, Rui; Yang, Zhijian; Luo, Xuming; Yu, Baoxi; Zhang, Jian; Fu, Ming"

作者地址："[Zhu, Weiwen; Yang, Zhijian; Luo, Xuming; Yu, Baoxi; Fu, Ming] Sun Yat Sen Univ, Affiliated Hosp 1, Dept Joint Surg, Guangzhou 510080, Peoples R China; [Wang, Rui] Chongqing Med Univ, Key Lab Lab Med Diagnost, Chinese Minist Educ, Chongqing 400016, Peoples R China; [Zhang, Jian] Chongqing Med Univ, Affiliated Hosp 1, Dept Orthoped, Chongqing 400016, Peoples R China"

通信作者："Fu, M (通讯作者)，Sun Yat Sen Univ, Affiliated Hosp 1, Dept Joint Surg, Guangzhou 510080, Peoples R China.; Zhang, J (通讯作者)，Chongqing Med Univ, Affiliated Hosp 1, Dept Orthoped, Chongqing 400016, Peoples R China."

来源：METABOLOMICS

ESI学科分类：BIOLOGY & BIOCHEMISTRY

WOS号：WOS:001078243000001

JCR分区：Q2

影响因子：3.5

年份：2023

卷号：19

期号：10

开始页：　

结束页：　

文献类型：Article

关键词："Metabolomics; GC-MS; Femur head necrosis; Linoleic acid metabolism; 9,12-Octadecadienoic acid"

摘要："IntroductionFemur head necrosis (FHN) is a challenging clinical disease with unclear underlying mechanism, which pathologically is associated with disordered metabolism. However, the disordered metabolism in cancellous bone of FHN was never analyzed by gas chromatography-mass spectrometry (GC-MS).ObjectivesTo elucidate altered metabolism pathways in FHN and identify putative biomarkers for the detection of FHN.MethodsWe recruited 26 patients with femur head necrosis and 22 patients with femur neck fracture in this study. Cancellous bone tissues from the femoral heads were collected after the surgery and were analyzed by GC-MS based untargeted metabolomics approach. The resulting data were analyzed via uni- and multivariate statistical approaches. The changed metabolites were used for the pathway analysis and potential biomarker identification.ResultsThirty-seven metabolites distinctly changed in FHN group were identified. Among them, 32 metabolites were upregulated and 5 were downregulated in FHN. The pathway analysis showed that linoleic acid metabolism were the most relevant to FHN pathology. On the basis of metabolites network, L-lysine, L-glutamine and L-serine were deemed as the junctions of the whole metabolites. Finally, 9,12-octadecadienoic acid, inosine, L-proline and octadecanoic acid were considered as the potential biomarkers of FHN.ConclusionThis study provides a new insight into the pathogenesis of FHN and confirms linoleic acid metabolism as the core."

基金机构：This work was supported by the Natural Science Foundation of Guangdong Province (Grant No. 2023A1515012682). The authors would like to thank Mr. Shengqiang Li for his help in sampling. [2023A1515012682]; Natural Science Foundation of Guangdong Province

基金资助正文：This work was supported by the Natural Science Foundation of Guangdong Province (Grant No. 2023A1515012682). The authors would like to thank Mr. Shengqiang Li for his help in sampling.