DNA methylation profiling and integrative multi-omics analysis of skin samples reveal important contribution of epigenetics and immune response in the pathogenesis of acne vulgaris

作者全名："Liu, Lin; Xue, Yuzhou; Chen, Jiayi; Li, Yuxin; Chen, Tingqiao; Pan, Xingyu; Zhong, Judan; Shao, Xinyi; Chen, Yangmei; Chen, Jin"

作者地址："[Liu, Lin; Chen, Jiayi; Li, Yuxin; Chen, Tingqiao; Pan, Xingyu; Zhong, Judan; Shao, Xinyi; Chen, Yangmei; Chen, Jin] Chongqing Med Univ, Affiliated Hosp 1, Dept Dermatol, Chongqing, Peoples R China; [Xue, Yuzhou] Peking Univ Third Hosp, Dept Cardiol, Beijing, Peoples R China; [Xue, Yuzhou] Peking Univ Third Hosp, Inst Vasc Med, Beijing, Peoples R China; [Chen, Yangmei; Chen, Jin] Chongqing Med Univ, Affiliated Hosp 1, Dept Dermatol, 1 Youyi Rd, Chongqing 400016, Peoples R China"

通信作者："Chen, YM; Chen, J (通讯作者)，Chongqing Med Univ, Affiliated Hosp 1, Dept Dermatol, 1 Youyi Rd, Chongqing 400016, Peoples R China."

来源：CLINICAL IMMUNOLOGY

ESI学科分类：IMMUNOLOGY

WOS号：WOS:001082499900001

JCR分区：Q2

影响因子：4.5

年份：2023

卷号：255

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Acne vulgaris; DNA methylation; Integrative multi-omics analysis; Multi-omics

摘要："The regulatory effect of DNA methylation on the pathogenesis of acne vulgaris is completely unknown. Herein we analyzed the DNA methylation profile in skin samples of acne vulgaris and further integrated it with gene expression profiles and single-cell RNA-sequencing data. Finally, 31,134 differentially methylated sites and 770 differentially methylated and expressed genes (DMEGs) were identified. The multi-omics analysis suggested the importance of DNA methylation in inflammation and immunity in acne. And DMEGs were verified in an external dataset and were closely related to early inflammatory acne. Additionally, we conducted experiments to verify the mRNA expression and DNA methylation level of DMEGs. This study supports the significant contribution of epigenetics to the pathogenesis of acne vulgaris and may provide new ideas for the molecular mechanisms of and potential therapeutic strategies for acne vulgaris."

基金机构：National Natural Science Foundation of China [82073462]; [GSE53795]; [GSE108110]; [GSE193973]

基金资助正文："This work was supported by the National Natural Science Foundation of China [grant numbers 82073462] ; and we are very grateful to the authors of the GSE53795, GSE108110, and GSE193973 datasets."