Identification of Plasma Inflammatory Markers of Adolescent Depression Using the Olink Proteomics Platform

作者全名："Yang, Ling; Cao, Maolin; Tian, Jing; Cui, Peijin; Ai, Ling; Li, Xue; Li, Hua; Gao, Menghan; Fang, Liang; Zhao, Libo; Gong, Fang; Zhou, Chanjuan"

作者地址："[Yang, Ling; Tian, Jing; Cui, Peijin; Gao, Menghan; Fang, Liang; Zhao, Libo; Zhou, Chanjuan] Chongqing Med Univ, Dept Neurol, Yongchuan Hosp, Chongqing, Peoples R China; [Yang, Ling; Fang, Liang; Zhao, Libo; Gong, Fang] Chongqing Key Lab Cerebrovasc Dis Res, Chongqing, Peoples R China; [Cao, Maolin; Ai, Ling; Zhou, Chanjuan] Chongqing Med Univ, Dept Gen Practice, Yongchuan Hosp, Chongqing, Peoples R China; [Li, Xue; Zhou, Chanjuan] Chongqing Med Univ, Yongchuan Hosp, Cent Lab, Chongqing, Peoples R China; [Li, Hua] Chongqing Med Univ, Dept Ophthalmol, Yongchuan Hosp, Chongqing, Peoples R China; [Fang, Liang; Gong, Fang; Zhou, Chanjuan] Chongqing Clin Res Ctr Geriatr Dis, Chongqing, Peoples R China; [Gong, Fang; Zhou, Chanjuan] Chongqing Med Univ, Yongchuan Hosp, 439 Xuanhua Rd, Chongqing 402160, Peoples R China"

通信作者："Gong, F; Zhou, CJ (通讯作者)，Chongqing Med Univ, Yongchuan Hosp, 439 Xuanhua Rd, Chongqing 402160, Peoples R China."

来源：JOURNAL OF INFLAMMATION RESEARCH

ESI学科分类：IMMUNOLOGY

WOS号：WOS:001085269800001

JCR分区：Q2

影响因子：4.2

年份：2023

卷号：16

期号：　

开始页：4489

结束页：4501

文献类型：Article

关键词：olink proximity extension assay; biomarkers; adolescent depression; inflammation

摘要："Purpose: The quality of life of worldwide adolescents has been seriously affected by depression. Notably, the inflammatory response is closely associated with the pathophysiology of depression. The present study applied a novel targeted proteomics technology, Olink proximity extension assay (PEA), to profile circulating immune-related proteins in adolescents with depression.Methods: In the present study, the expression levels of 92 inflammation-related proteins were compared between adolescents with depression (ADs) (n=15) and healthy controls (HCs) (n=15), using the OLINK PEA inflammation panel. We further validated 5 top proteins that were identified through KEGG and GO analyses between 40 HCs and 50 ADs, including CCL4, CXCL5, CXCL6, CXCL11, and IL-18 using enzyme linked immunosorbent assay (ELISA).Results: We identified 13 differentially expressed proteins between the two cohorts, including 5 up-regulated and 8 down-regulated proteins. Among them, the TRAIL protein levels were significantly negatively correlated with the HAMA-14 score (r=-0.538, p= 0.038), and the levels of transforming growth factor alpha (TGF-alpha) were significantly associated with a change in appetite (r =-0.658, p = 0.008). After validation by ELISA, CCL4, CXCL5, CXCL11, and IL-18 showed significant changes between ADs and HCs (p < 0.05), while CXCL6 showed an up-regulated tendency in ADs (p=0.0673). The pooled diagnostic efficacy (area under the curve [AUC]) of these five inflammation markers in clinical diagnosis for adolescent depression was 0.819 (95% CI: 0.735-0.904).Conclusion: We report a number of inflammation-related plasma biomarkers, which uncover a potential involvement of chemokines, cytokines, and cytokine receptors in adolescent depression. Their roles in the pathophysiology of depression need to be further elucidated."

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