Time-restricted feeding affects the fecal microbiome metabolome and its diurnal oscillations in lung cancer mice

作者全名："Fang, Gaofeng; Wang, Shengquan; Chen, Qianyao; Luo, Han; Lian, Xuemei; Shi, Dan"

作者地址："[Fang, Gaofeng; Wang, Shengquan; Chen, Qianyao; Luo, Han; Lian, Xuemei; Shi, Dan] Chongqing Med Univ, Sch Publ Hlth, Dept Nutr & Food Hyg, Chongqing 400016, Peoples R China; [Fang, Gaofeng; Wang, Shengquan; Chen, Qianyao; Luo, Han; Lian, Xuemei; Shi, Dan] Chongqing Med Univ, Ctr Lipid Res, Key Lab Mol Biol Infect Dis, Minist Educ, Chongqing 400016, Peoples R China"

通信作者："Lian, XM; Shi, D (通讯作者)，Chongqing Med Univ, Sch Publ Hlth, Dept Nutr & Food Hyg, Chongqing 400016, Peoples R China."

来源：NEOPLASIA

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:001103704500001

JCR分区：Q1

影响因子：6.3

年份：2023

卷号：45

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Time-restricted feeding; Circadian rhythm; Gut microbiota; Metabolome; Lung cancer

摘要："The homeostasis of the gut microbiota and circadian rhythm is critical to host health, and both are inextricably intertwined with lung cancer. Although time-restricted feeding (TRF) can maintain circadian synchronization and improve metabolic disorders, the effects of TRF on the fecal microbiome, metabolome and their diurnal oscillations in lung cancer have not been discussed. We performed 16S rRNA sequencing and untargeted metabonomic sequencing of the feces prepared from models of tumor-bearing BALB/c nude mice and urethaneinduced lung cancer. We demonstrated for the first time that TRF significantly delayed the growth of lung tumors. Moreover, TRF altered the abundances of the fecal microbiome, metabolome and circadian clocks, as well as their rhythmicity, in lung cancer models of tumor-bearing BALB/c nude mice and/or urethane-induced lung cancer C57BL/6J mice. The results of fecal microbiota transplantation proved that the antitumor effects of TRF occur by regulating the fecal microbiota. Notably, Lactobacillus and Bacillus were increased upon TRF and were correlated with most differential metabolites. Pathway enrichment analysis of metabolites revealed that TRF mainly affected immune and inflammatory processes, which might further explain how TRF exerted its anticancer benefits. These findings underscore the possibility that the fecal microbiome/metabolome regulates lung cancer following a TRF paradigm."

基金机构：National Natural Science Founda-tion of China [8210120502]; China Postdoctoral Science Foundation [2021M693755]; CQMU Program for Youth Innovation in Future Medicine [W0085]

基金资助正文："This research was funded by the National Natural Science Founda-tion of China (No. 8210120502) , China Postdoctoral Science Foundation (2021M693755) , and CQMU Program for Youth Innovation in Future Medicine (No. W0085) ."