Multi-parameter cardiac magnetic resonance imaging detects anthracycline-induced cardiotoxicity in rabbits model

作者全名："Hu, Yurou; Ma, Peisong; Chen, Lin; Liu, Juan; Shang, Yongning; Jian, Wang"

作者地址："[Hu, Yurou; Chen, Lin; Jian, Wang] Third Mil Med Univ, Army Med Univ, Southwest Hosp, Dept Radiol, Chongqing, Peoples R China; [Ma, Peisong] Chongqing Med Univ, Affiliated Hosp 1, Dept Radiol, Chongqing, Peoples R China; [Liu, Juan; Shang, Yongning] Third Mil Med Univ, Army Med Univ, Southwest Hosp, Dept Ultrasound, Chongqing, Peoples R China; [Hu, Yurou; Chen, Lin; Jian, Wang] Third Mil Med Univ, Army Med Univ, Southwest Hosp, 7T Magnet Resonance Imaging Translat Med Ctr, Chongqing, Peoples R China"

通信作者："Jian, W (通讯作者)，Third Mil Med Univ, Army Med Univ, Southwest Hosp, Dept Radiol, Chongqing, Peoples R China.; Shang, YN (通讯作者)，Third Mil Med Univ, Army Med Univ, Southwest Hosp, Dept Ultrasound, Chongqing, Peoples R China.; Jian, W (通讯作者)，Third Mil Med Univ, Army Med Univ, Southwest Hosp, 7T Magnet Resonance Imaging Translat Med Ctr, Chongqing, Peoples R China."

来源：HELIYON

ESI学科分类：　

WOS号：WOS:001123481100001

JCR分区：Q1

影响因子：3.4

年份：2023

卷号：9

期号：11

开始页：　

结束页：　

文献类型：Article; Early Access

关键词：T1 and T2 mapping; CMR; Anthracycline-induced cardiotoxicity; Animal models

摘要："Background: Cardiac magnetic resonance (CMR) quantitative T1 and T2 mapping offers a non-invasive means to evaluate early cardiotoxicity changes. This study aimed to pinpoint the earliest CMR indicators of myocardial injury in Anthracycline-induced cardiotoxicity (AIC) and to elucidate the connections between these CMR indicators and associated pathological indicators.Methods: A total of 34 rabbits were administered doxorubicin at a dosage of 1 mg/kg/weekly. The study incorporated six 3T CMR scan time points: baseline, and at intervals of four, six, eight, twelve, and sixteen weeks. Cine, T1 and T2 mapping sequences assessed the left ventricular ejection fraction (LVEF), native T1, extracellular volume fraction (ECV), and T2 values. Following each time point, three rabbits were sacrificed for histological analysis involving Hematoxylin and eosin (H&E), Masson, TUNEL, and microvascular density (MVD) stains. Spearman correlations and linear mixed model analysis served in the statistical analysis.Results: Diverse degrees of alternation were recorded in LVEF, native T1, T2, and ECV over time. LVEF declined to 49.0 +/- 2.6 % at 12 weeks from the baseline of 53.4 +/- 3.2 %, p < 0.001. Native T1 values increase from the baseline (1396.5 +/- 79.2 ms) until 8 weeks (1498.8 +/- 95.4 ms, p < 0.001). T2 values increased from the baseline (36.6 +/- 3.3 ms) within 4 weeks of initiation (37.5 +/- 3.4, p = 0.02) and remained elevated through 16 weeks (42.8 +/- 0.3, p < 0.01). ECV was elevated at 8 weeks (33.9 +/- 3.8 %, p = 0.005) compared to the baseline (30.2 +/- 2.5 %). By week 12, myocardial edema and increased CVF were apparent (p = 0.04 and = 0.001, respectively). The area under ROC curve for positive CMR presence and the gold standards were 0.87 (T2-ROC, 4 weeks) and 0.92 (LVEF&BNP-ROC, 12 weeks).Conclusion: T1 and T2 mapping are effective tools for cardiotoxicity detection and monitoring. The prolongation of T2 value emerged as the most consistent and early-onset indicator."

基金机构：National Natural Science Foundation of China [81971587]; National InnovationTalent Promotion Program [4139Z2399]; Natural Science Foundation of Chongqing [cstc2020jcyj-msxmX0399]; Chongqing postdoctoral Science Foundation [CSTB2022BSXM-JCX0032]

基金资助正文："This work was funded by the National Natural Science Foundation of China (grant number 81971587) , National InnovationTalent Promotion Program (4139Z2399) , the Natural Science Foundation of Chongqing (grant number cstc2020jcyj-msxmX0399) , and Chongqing postdoctoral Science Foundation (grant number CSTB2022BSXM-JCX0032) ."