Urease-Powered Micromotors with Spatially Selective Distribution of Enzymes for Capturing and Sensing Exosomes

作者全名："Liu, Xiaoxia; Wang, Yong; Peng, Yixin; Shi, Jinjin; Chen, Wenjun; Wang, Wei; Ma, Xing"

作者地址："[Liu, Xiaoxia; Peng, Yixin; Chen, Wenjun; Wang, Wei; Ma, Xing] Harbin Inst Technol Shenzhen, Sch Mat Sci & Engn, Shenzhen 518055, Guangdong, Peoples R China; [Liu, Xiaoxia; Chen, Wenjun; Ma, Xing] Harbin Inst Technol Shenzhen, Sauvage Lab Smart Mat, Shenzhen 518055, Guangdong, Peoples R China; [Wang, Yong] Chongqing Med Univ, Coll Lab Med, Key Lab Clin Lab Diagnost, Minist Educ, Chongqing 400016, Peoples R China; [Shi, Jinjin] Zhengzhou Univ, Sch Pharmaceut Sci, Zhengzhou 450001, Peoples R China"

通信作者："Chen, WJ; Wang, W; Ma, X (通讯作者)，Harbin Inst Technol Shenzhen, Sch Mat Sci & Engn, Shenzhen 518055, Guangdong, Peoples R China.; Chen, WJ; Ma, X (通讯作者)，Harbin Inst Technol Shenzhen, Sauvage Lab Smart Mat, Shenzhen 518055, Guangdong, Peoples R China."

来源：ACS NANO

ESI学科分类：MATERIALS SCIENCE

WOS号：WOS:001124595700001

JCR分区：Q1

影响因子：15.8

年份：2023

卷号：17

期号：23

开始页：24343

结束页：24354

文献类型：Article

关键词：enzymatic micromotors; spatially distribution; flow field; driving module; functional module

摘要："Enzyme-catalyzed micro/nanomotors (MNMs) exhibit tremendous potential for biological isolation and sensing, because of their biocompatibility, versatility, and ready access to biofuel. However, flow field generated by enzyme-catalyzed reactions might significantly hinder performance of surface-linked functional moieties, e.g., the binding interaction between MNMs and target cargos. Herein, we develop enzymatic micromotors with spatially selective distribution of urease to enable the independent operation of various modules and facilitate the capture and sensing of exosomes. When urease is modified into the motors' cavity, the flow field from enzyme catalysis has little effect on the exterior surface of the motors. The active motion and encapsulating urease internally result in enhancement of similar to 35% and 18% in binding efficiency of target cargos, e.g., exosomes as an example here, compared to their static counterparts and moving micromotors with urease modified externally, respectively. Once exosomes are trapped, they can be transferred to a clean environment by the motors for Raman signal detection and/or identification using the surface Raman enhancement scattering (SERS) effect of coated gold nanoshell. The biocatalytic micromotors, achieving spatial separation between driving module and function module, offer considerable promise for future design of multifunctional MNMs in biomedicine and diagnostics."

基金机构："National Natural Science Foundation of China [52072095, 52202347, T2322006]; National Natural Science Foundation of China [KQTD20170809110344233, RCBS20210609103646022, RCYX20210609103122038]; Shenzhen Science and Technology Program [2021A1515110272]; Guangdong Basic and Applied Basic Research Foundation [2023NSCQ-MSX1158]; Natural Science Foundation of Chongqing, China"

基金资助正文："The authors thank the financial support from National Natural Science Foundation of China (Nos. 52072095, 52202347, T2322006), Shenzhen Science and Technology Program (Nos. KQTD20170809110344233, RCBS20210609103646022, RCYX20210609103122038), Guangdong Basic and Applied Basic Research Foundation (No. 2021A1515110272), and Natural Science Foundation of Chongqing, China (No. 2023NSCQ-MSX1158)."