Transcriptome-Wide Dynamics of m7G-Related LncRNAs during the Progression from HBV Infection to Hepatocellular Carcinoma

作者全名："Shi, Min; Zhu, Shunshun; Sun, Linying; Hu, Jieli; Li, Hao; Dai, Wenqing; Song, Ning; Li, Minmin; Wu, Ying; Xu, Donghua; Guo, Tao"

作者地址："[Shi, Min; Zhu, Shunshun; Guo, Tao] Weifang Med Univ, Sch Basic Med Sci, Dept Pathophysiol, Weifang 261053, Shandong, Peoples R China; [Sun, Linying] Weifang Med Univ, Sch Basic Med Sci, Dept Funct Lab, Weifang 261053, Shandong, Peoples R China; [Hu, Jieli] Chongqing Med Univ, Key Lab Mol Biol Infect Dis, Minist Educ, Chongqing 400016, Peoples R China; [Li, Hao] First Affiliated Hosp Weifang Med Univ, Hepatobiliary & Pancreat Med Ctr, Weifang 261000, Shandong, Peoples R China; [Dai, Wenqing; Xu, Donghua] First Affiliated Hosp Weifang Med Univ, Cent Lab, Weifang 261000, Shandong, Peoples R China; [Song, Ning; Li, Minmin] Weifang Med Univ, Sch Stomatol, Weifang 261053, Shandong, Peoples R China; [Wu, Ying] Guangxi Med Univ, Liuzhou Peoples Hosp, Liuzhou Key Lab Infect Dis & Immunol, Guangxi Hlth Commiss Key Lab Clin Biotechnol, Liuzhou 545006, Guangxi, Peoples R China"

通信作者："Guo, T (通讯作者)，Weifang Med Univ, Sch Basic Med Sci, Dept Pathophysiol, Weifang 261053, Shandong, Peoples R China.; Xu, DH (通讯作者)，First Affiliated Hosp Weifang Med Univ, Cent Lab, Weifang 261000, Shandong, Peoples R China."

来源：FRONTIERS IN BIOSCIENCE-LANDMARK

ESI学科分类：　

WOS号：WOS:001146038600014

JCR分区：Q2

影响因子：3.3

年份：2023

卷号：28

期号：12

开始页：　

结束页：　

文献类型：Article

关键词：HBV-related HCC; lncRNA; m7G

摘要："Background: The functional ramifications of internal N7-methylguanosine (m7G) modification on RNAs have recently come to light, yet its regulatory influence on long noncoding RNAs (lncRNAs) during the inflammatory-carcinogenesis transformation process in hepatitis passing HBV-related HCC, comprising both HCC tissue (tumor group, HBV+) and corresponding adjacent liver tissue (paracancerous group, HBV+), were collected for analysis. Additional adjacent normal liver tissues (normal group, HBV-) were acquired from patients with hepatic hemangioma, serving as controls. Employing MeRIP-seq, differential m7G levels of lncRNAs across these groups were compared to identify a subset of lncRNAs exhibiting continuous and dynamic changes in m7G modification. Subsequently, in vitro validation was conducted. Results: A total of 856 lncRNAs exhibited alterations in m7G modification when compared to paracancerous tissue and normal tissue. Similarly, 1775 lncRNAs displayed changes in m7G modification when comparing HCC tissue to paracancerous tissue. For intergroup comparison, orthogonal analysis revealed that 6 lncRNAs consistently demonstrated hyper-m7G modification. In vitro validation confirmed that among these 6 lncRNAs, TEKT4P2 and DNM1P41 exhibited m7G modification-dependent expression. Conclusions: This study provides a comprehensive analysis of lncRNA m7G modification during the inflammatory-carcinogenesis transformation process in HBV-mediated HCC. The findings highlight the potential for multiple lncRNAs to undergo m7G modification changes, with TEKT4P2 and DNM1P41 identified as promising molecular targets within this intricate regulatory landscape."

基金机构：Natural Science Foundation of Shandong Province [ZR2021QH200]; Project of Shandong Province Youth In- novation Team [2022KJ267]; Research Start-up Funds of Weifang Medical University [04102001]

基金资助正文："Funding This study was supported by Natural Science Foundation of Shandong Province (Grant numbers: ZR2021QH200) , Project of Shandong Province Youth In- novation Team (Grant numbers: 2022KJ267) and Research Start-up Funds of Weifang Medical University (Grant numbers: 04102001) ."