Identification of EMT-associated prognostic features among grade II/III gliomas

作者全名："Yang, Wenyong; Lin, Liangbin; Lu, Tianqi; Yu, Hui; Zhang, Sunfu"

作者地址："[Yang, Wenyong; Lin, Liangbin; Lu, Tianqi; Yu, Hui; Zhang, Sunfu] Chongqing Med Univ, Chengdu Hosp 2, Affiliated Hosp,Southwest Jiaotong Univ,Dept Urol, Med Res Ctr,Peoples Hosp Chengdu 3,Dept Neurosurg, Chengdu, Peoples R China; [Lu, Tianqi] Southwest Jiaotong Univ, Peoples Hosp Chengdu 3, Ctr Gastrointestinal & Minimally Invas Surg, Dept Gen Surg,Affiliated Hosp, Chengdu, Peoples R China; [Lu, Tianqi] Southwest Jiaotong Univ, Peoples Hosp Chengdu 3, Dept Gen Surg,Affiliated Hosp, Obes & Metab Med Engn Integrat Lab, Chengdu, Sichuan, Peoples R China"

通信作者："Zhang, SF (通讯作者)，Chongqing Med Univ, Chengdu Hosp 2, Affiliated Hosp,Southwest Jiaotong Univ,Dept Urol, Med Res Ctr,Peoples Hosp Chengdu 3,Dept Neurosurg, Chengdu, Peoples R China."

来源：SCIENTIFIC REPORTS

ESI学科分类：Multidisciplinary

WOS号：WOS:001156600900001

JCR分区：Q1

影响因子：4.6

年份：2024

卷号：14

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：　

摘要："Grade II/III gliomas have a highly heterogeneous clinical course. Identifying prognostic biomarkers in grade II/III gliomas is essential to guide clinical management. We explored epithelial-mesenchymal transition (EMT)-related genes to uncover prognostic features in grade II/III gliomas. Consensus cluster analysis of 200 EMT-related genes classified 512 grade II/III glioma samples into two molecular subtypes, C1 and C2. The C1 subtype had significantly worse overall survival compared to the C2 subtype. Pathway analysis revealed C1 tumors were highly associated with tumor progression pathways and demonstrated higher immune cell infiltration scores. Differential expression analysis identified four genes (ACTN1, AQP1, LAMC3, NRM) that discriminated the two subtypes. Validation in external datasets confirmed that high expression of this four-gene signature predicted poor prognosis in grade II/III gliomas. Cellular experiments showed ACTN1, AQP1 and NRM promoted glioma cell proliferation, migration and invasion. We examined correlations of the signature genes with T cell exhaustion markers and found ACTN1 expression had the strongest association. Immunohistochemistry analysis further demonstrated that ACTN1 protein expression in grade II/III gliomas was negatively correlated with patient overall survival. In summary, our study identified a concise four-gene signature that robustly predicts grade II/III gliomas prognosis across multiple datasets. The signature provides clinical relevance in distinguishing more aggressive grade II/III glioma tumors. Targeting the ACTN1, AQP1 and NRM genes may offer new therapeutic opportunities to improve grade II/III gliomas patient outcomes."

基金机构：Sichuan Science and Technology Department

基金资助正文：No Statement Available