Antiplatelet Agents Inhibit Platelet Adhesion and Aggregation on Glass Surface Under Physiological Flow Conditions: Toward a Microfluidic Platelet Functional Assay Without Additional Adhesion Protein Modification

作者全名："Liu, Zhanshu; Huang, Xiaojing; Gao, Xuemei; Zhang, Tiancong; He, Cui; Ding, Ling; Li, Yuan"

作者地址："[Liu, Zhanshu] Chongqing Med Univ, Yongchuan Hosp, Dept Hematol, Chongqing, Peoples R China; [Huang, Xiaojing; Gao, Xuemei; Zhang, Tiancong; He, Cui; Li, Yuan] Chongqing Med Univ, Yongchuan Hosp, Cent Lab, Chongqing, Peoples R China; [Ding, Ling] Yongchuan Sub Ctr, Chongqing Blood Ctr, Chongqing, Peoples R China; [Li, Yuan] Chongqing Med Univ, Cent Lab Yongchuan Hosp, 439 Xuanhua Rd, Chongqing 402160, Peoples R China"

通信作者："Li, Y (通讯作者)，Chongqing Med Univ, Cent Lab Yongchuan Hosp, 439 Xuanhua Rd, Chongqing 402160, Peoples R China."

来源：JOURNAL OF CARDIOVASCULAR PHARMACOLOGY

ESI学科分类：PHARMACOLOGY & TOXICOLOGY

WOS号：WOS:001158664600003

JCR分区：Q2

影响因子：2.6

年份：2024

卷号：83

期号：2

开始页：173

结束页：182

文献类型：Article

关键词：microfluidic chip; flow conditions; antiplatelet drugs; platelet adhesion and aggregation; glass

摘要："As the pathogenesis of arterial thrombosis often includes platelet adhesion and aggregation, antiplatelet agents are commonly used to prevent thromboembolic events. Here, a new microfluidic method without additional adhesion protein modification was developed to quantify the inhibitory effect of antiplatelet drugs on the adhesion and aggregation behavior of platelets on glass surfaces under physiological flow conditions. Polydimethylsiloxane-glass microfluidic chips were fabricated by soft photolithography. Blood samples from healthy volunteers or patients before and after taking antiplatelet drugs flowed through the microchannels at wall shear rates of 300 and 1500 second-1, respectively. The time to reach 2.5% platelet aggregation surface coverage (Ti), surface coverage (A150s), and mean fluorescence intensity (F150s) were used as quantitative indicators. Aspirin (80 mu M) prolonged Ti and reduced F150s. Alprostadil, ticagrelor, eptifibatide, and tirofiban prolonged Ti and reduced A150s and F150s in a concentration-dependent manner, whereas high concentrations of alprostadil did not completely inhibit platelet aggregation. Aspirin combined with ticagrelor synergistically inhibited platelet adhesion and aggregation; GPIb-IX-von Willebrand factor inhibitors partially inhibited platelet aggregation, and the inhibition was more pronounced at 1500 than at 300 second-1. Patient administration of aspirin or (and) clopidogrel inhibited platelet adhesion and aggregation on the glass surface under flow conditions. This technology is capable of distinguishing the pharmacological effects of various antiplatelet drugs on inhibition of platelet adhesion aggregation on glass surface under physiological flow conditions, which providing a new way to develop microfluidic platelet function detection method without additional adhesive protein modification for determining the inhibitory effects of antiplatelet drugs in the clinical setting."

基金机构：National Nature Sciences Foundation of China [11702047]; Technological Innovation Program for Society and Livelihood Guarantee of Chongqing [cstc2017shmsA130009]; Joint project of Chongqing Health Commission and Science and Technology Bureau [2023GDRC008]; Postgraduate Innovation Fund of Yongchuan Hospital Affiliated to Chongqing Medical University [YJSCX202202]

基金资助正文："Supported by the National Nature Sciences Foundation of China (11702047),the Technological Innovation Program for Society and Livelihood Guarantee of Chongqing (cstc2017shmsA130009), a Joint project of Chongqing Health Commission and Science and Technology Bureau(2023GDRC008), and the Postgraduate Innovation Fund of Yongchuan Hospital Affiliated to Chongqing Medical University (YJSCX202202).Funding sources were not involved in the study design, in the collection, analysis, and interpretation of data, in the writing of the report, and in thedecision to submit the article for publication."